SKU: 49219632772

Human IFN-γ OneStep OptiQuant ELISA Kit

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Description

Human IFN-γ OneStep OptiQuant ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 4. 0% Inter assay: 4. 5% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 2. 577pg ml Range 15. 63 pg mL 1000 pg mL Recovery Cell culture supernatant: 93% Serum: 109% Plasma: 111% Assay time 45 minutes

Product Specification


Antigen IFN-γ
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage

2 to 8 °C as supplied.

Kit


Precision Intra-assay: 4.0%;
Inter-assay: 4.5%
Sample type Cell culture supernatant, Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 2.577pg/ml
Range 15.63 pg/mL – 1000 pg/mL
Recovery Cell culture supernatant: 93%
Serum: 109%
Plasma: 111%
Assay time 45 minutes
Species reactivity Human
NIBSC convert

The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).

Background

Interferon-gamma (IFN-γ) is a dimeric cytokine central to innate and adaptive immunity, primarily produced by activated T cells and natural killer (NK) cells. Structurally, IFN-γ exists as a homodimer, with each subunit comprising six α-helical domains stabilized by intra- and interchain disulfide bonds. It signals through a heterodimeric receptor complex composed of IFNGR1 and IFNGR2, initiating JAK-STAT pathway activation. Post-translational glycosylation at asparagine residues (e.g., Asn25 and Asn97 in humans) enhances its stability and modulates receptor interaction dynamics. Immune Regulatory Roles and Therapeutic Cross-Talk of IFN-γ: Interferon-gamma (IFN-γ) orchestrates multifaceted immune responses, exerting critical antiviral and antitumor defense by inducing cellular antiviral states and enhancing tumor immunosurveillance through upregulation of MHC class I/II expression. It activates macrophages to bolster phagocytosis, reactive oxygen species production, and pathogen clearance, while polarizing CD4+ T cells into Th1 subsets to amplify cell-mediated immunity. Despite its protective roles, IFN-γ exhibits a dual nature in inflammation, where excessive levels contribute to autoimmune disorders (e.g., rheumatoid arthritis, multiple sclerosis) and chronic inflammatory pathologies. Therapeutically, IFN-γ synergizes with proinflammatory cytokines like TNF-α and IL-12 to amplify immune activation, while counteracting the immunosuppressive effects of IL-4 and IL-10, highlighting its pivotal yet complex role in balancing immune defense and inflammatory regulation. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IFN-γ quantification in time-sensitive or resource-limited settings.

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Exchange/Return Notes
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SKU: 49219632772

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Citasio
New York, US
★★★★★ 5
Great product
Size: 12 fl. oz. (Pack of 1)
Noticeable difference in the first 50 miles .
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Reviewed in the United States on March 30, 2026
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Stephen Capizzi
Chelsea, US
★★★★★ 5
Great product!
Size: 12 fl. oz. (Pack of 1)
Would recommend pouring oil into container after pouring in and shaking it up, great additive recommended by some old folks but I would have to say it’s just thick as molasses. Used in 2016 FORD F150 5.0 V8
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Reviewed in the United States on April 3, 2025
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F.P.
Port Orchard, US
★★★★★ 5
Great product!
Size: 12 fl. oz. (Pack of 1)
My Dad believed in Bardaul and I do to!
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Reviewed in the United States on January 22, 2025
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Roberto Heredia
Boise, US
★★★★★ 5
Inigualable protector del motor
Size: 12 fl. oz. (Pack of 1)
Producto extraordinario para el motor
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Reviewed in the United States on April 26, 2024
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NickU179
Los Angeles, US
★★★★★ 5
Great for High Mileage vehicles
Size: 12 fl. oz. (Pack of 1)
Added into my 2014 Dodge Grand Caravan with 100k miles before a family trip and eliminated knock, slow starts and improved our gas mileage by about 10% I think! super easy to add to the oil and very happy with results.
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Reviewed in the United States on April 18, 2024

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