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Description
Rat IgA OneStep ELISA KitProduct Specification Antigen IgA Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Rt Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 5. 7% Inter assay: 7. 1% Sample type Cell culture supernatant, Serum, Plasm Assay type Sandwich (quantitative) Sensitivity 0. 043 ng ml Range 1. 56 ng mL 100ng mL Recovery Cell culture supernatant: 99% Serum: 90% Plasm: 115% Assay time 60 minutes Species
Product Specification
| Antigen | IgA |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 5.7%; Inter-assay: 7.1% |
| Sample type | Cell culture supernatant, Serum, Plasm |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.043 ng/ml |
| Range | 1.56 ng/mL – 100ng/mL |
| Recovery | Cell culture supernatant: 99% Serum: 90% Plasm: 115% |
| Assay time | 60 minutes |
| Species reactivity | Rat |
Background
Immunoglobulin A (IgA) is a pivotal antibody isotype central to mucosal immunity and systemic immune regulation in rats. Structurally, rat IgA exists predominantly as a monomer in serum and as a dimer or polymer (secretory IgA, sIgA) at mucosal surfaces. Each IgA molecule comprises two α-heavy chains and two light chains (κ or λ), forming a flexible Y-shaped structure stabilized by disulfide bonds. The α-heavy chain contains three constant domains (Cα1–Cα3), with the Fc region (Cα2–Cα3) facilitating interactions with the Fcα receptor (FcαRI) and the polymeric immunoglobulin receptor (pIgR). Secretory IgA incorporates a J-chain and a secretory component, which enhance its stability in protease-rich mucosal environments. Extensive glycosylation of rat IgA further modulates its solubility, receptor binding, and resistance to enzymatic degradation. Rat IgA is the primary antibody safeguarding mucosal surfaces (e.g., respiratory, gastrointestinal tracts), where it neutralizes pathogens, blocks microbial adhesion, and maintains commensal microbiota balance. Secretory IgA mediates immune exclusion by trapping pathogens in mucus for mechanical clearance, while serum IgA contributes to systemic immune homeostasis by regulating inflammatory responses via FcαRI signaling. Additionally, rat IgA may engage in eosinophil-mediated antiparasitic defense and modulate allergic or autoimmune reactions through interactions with immune cells. Rat IgA shares conserved structural motifs with murine and human IgA, particularly in the α-chain constant domains. However, species-specific variations in glycosylation patterns, J-chain assembly, and secretory component binding necessitate species-specific assays for accurate detection. For example, rat IgA lacks the extended hinge region characteristic of human IgA1, resulting in distinct antigen accessibility and protease resistance. Clinical and Experimental Significance of IgA Levels: Elevated IgA: Associated with chronic mucosal infections (e.g., intestinal pathogens), autoimmune disorders (e.g., experimental colitis models), or inflammatory conditions. Reduced IgA: May indicate primary immunodeficiency (e.g., selective IgA deficiency in genetically modified models), immunosuppressive therapies, or gut dysbiosis. Applications of the Rat IgA ELISA Kit: This OneStep Rat IgA ELISA kit is designed for rapid and precise quantification of IgA in serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgA quantification in time-sensitive or resource-limited settings.
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