SKU: 3517616833

Human Serum Albumin Competitive OneStep ELISA Kit

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Description

Human Serum Albumin Competitive OneStep ELISA KitProduct Specification Antigen Serum Albumin Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 3. 7% Inter assay: 7. 4% Sample type Cell culture supernatant, Serum,Plasma Assay type Competitive (quantitative) Sensitivity 3. 56 ng ml Range 39. 06 ng mL 2500 ng mL Recovery Cell culture supernatant: 100% Serum: 95% Plasma: 96% Assay time 60

Product Specification


Antigen Serum Albumin
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage 2 to 8 °C as supplied.

Kit


Precision Intra-assay: 3.7%;
Inter-assay: 7.4%
Sample type Cell culture supernatant, Serum,Plasma
Assay type Competitive (quantitative)
Sensitivity 3.56 ng/ml
Range 39.06 ng/mL – 2500 ng/mL
Recovery Cell culture supernatant: 100%
Serum: 95%
Plasma: 96%
Assay time 60 minutes
Species reactivity Human

Background

Human Serum Albumin (HSA) is a single polypeptide chain composed of 585 amino acids, with a molecular weight of ~66.5 kDa. It is rich in cysteine residues (17 disulfide bonds) and lacks carbohydrates, making it highly soluble and stable. The protein folds into a heart-shaped tertiary structure with three homologous domains (I, II, and III), each divided into two subdomains (A and B). The structure is stabilized by disulfide bonds and hydrophobic interactions. HSA has multiple ligand-binding sites, notably for fatty acids, hormones, and drugs, located in hydrophobic pockets and flexible loops. HAS is a vital multifunctional plasma protein critical for homeostasis. It maintains ~80% of plasma colloid osmotic pressure to prevent vascular fluid leakage, transports hydrophobic molecules (e.g., fatty acids, hormones, drugs) and metal ions (Ca²⁺, Cu²⁺), and exerts antioxidant activity via its Cys-34 thiol group to neutralize free radicals and bind pro-oxidant metals. HSA also buffers blood pH using histidine residues and supports endothelial stability by regulating vascular permeability and nitric oxide bioavailability, thereby modulating blood flow. These roles collectively highlight HSA’s indispensable contribution to systemic health and disease regulation. HSA exhibits significant evolutionary conservation, sharing high sequence homology with serum albumin in other mammals. For instance, it shows ~76% sequence identity with Bovine Serum Albumin (BSA) and ~78% identity with Mouse Serum Albumin, highlighting its structural and functional preservation across species. This homology extends to its three-domain architecture (domains I, II, and III) and the characteristic disulfide bond patterns, both of which are remarkably conserved. low levels, caused by reduced synthesis (e.g., liver cirrhosis), excessive loss (e.g., nephrotic syndrome), malnutrition, or inflammation (e.g., sepsis), leads to edema, ascites, impaired drug efficacy, and poorer outcomes in critical illnesses. Conversely, elevated levels, typically due to dehydration (hemoconcentration) or rare genetic factors, is often transient but may signal chronic inflammation or metabolic disorders if persistently elevated. Applications of the Human Serum Albumin Competitive OneStep ELISA Kit: Human Serum Albumin Competitive OneStep ELISA kit enables rapid, accurate quantification of Serum Albumin in serum, plasma, or cell culture supernatants. Advantages of this Competitive OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Serum Albumin quantification in time-sensitive or resource-limited settings.

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SKU: 3517616833

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4.8 ★★★★★
Based on 8 reviews
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Product Reviews
S
Verified Purchase
SP
Belleville, US
★★★★★ 4
Comfortable, affordable, and great for small spaces!
Color: Light Purple, Color: Light Purple
I had previously left a review of two stars, but have since deleted that review after I've owned the chair for a few weeks now. Initially, I stated the chair was uncomfortable and not cushioned at all. But this issue quickly resolved after sitting it for a longer time, it seems the cushion molds to you. So it may be uncomfortable the first few times sitting on it, but it eventually softens. The cushion is now soft yet sturdy. You don't sink in too much, but it's comfortable for sitting for long periods of time. I personally enjoy the mesh back as well, it supports my lower back and doesn't cause back pain. Normally I have to use a cushion of my own to support my lower back for most chairs, but this one actually supports me in a way that doesn't require an extra cushion - so that's really nice. The chair is fairly on the smaller end, which is perfect for low desks or tight spaces, which is exactly what I wanted. The height can be adjusted, but even at the highest setting, it is still fairly low. Keep that in mind - it may not be super comfortable for those over 6 ft. The arms flip up which makes storing it under a low desk very easy. The cushion is thick and very accurate to the picture. I love that it is lightweight and doesn't make much noise when rolling the chair. It also feels very sturdy. Some of the reviews say it feels flimsy, and I don't know if some are just assembling it incorrectly, because for me this thing is very strong and holds up quite nicely under my weight. Overall, this is exactly what I wanted for my personal living space. A chair that's not too bulky, but comfortable enough to sit in for long hours. It functions as advertised for this purpose. One final thing -- the color selection is gorgeous. I bought the light purple color and it fits my setup nicely! Color was accurate to the picture, and looks very cute and adorable in my space.
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Reviewed in the United States on December 21, 2025
C
Verified Purchase
Christine Franzone
Louisville, US
★★★★★ 5
Exteremly comfortable
Color: Dark Black
Reasonably priced, very comfortable l, easy assembly
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
J
Verified Purchase
James F Evans
West Palm Beach, US
★★★★★ 5
Surprisingly amazing chair!
Color: Pink, Color: Pink
tl;dr: If you need a chair that has the features of this one, then this one is a winner! Hits above its weight class for sure. My wife is 5’. She’s small. For whatever reason she ended up with a chair made for big people, like 6+ feet and up to 500lbs type of a chair. She’d often even sleep on it and chill on it. The chair was big enough to fit 2 of her to be honest. It was an expensive chair that I won at some raffle and didn’t like myself. Over the years it ripped (got a cover for it) and even started to get lopsided (from the sleeping) and finally a spring broke (hence the pillow on it in the pictures) She obviously lives pink. I also wanted to get a chair where she could work and not end ip asleep on. Not like we have a couch or a bed for that 😂. This chair is very obviously smaller than her previous chair, it’s also significantly cheaper in price and build, it’s also not as durable feeling or stable, and it’s just not as padded. Yet somehow it’s an amazing chair that we both like better than her previous chair??? Like it’s crazy that this chair somehow works better in so many ways. Her desk is modified to be shorter than normal desks (again, she small lol) and her previous chair was just too big. The wheels are also like SUPER smooth, like you can run a chair race easily with it. It rolls over her rug and tucks into her desk (which is in our bedroom) easily. The amount of space this has save us when the chair isn’t being used is a lot. Previous chair was much bigger and didn’t tuck in at all. The color is nice and the padding is plenty for a small and much lighter than 500lbs woman. I’m 6 feet and 200 lbs and the chair is nice for me as well. My chair is bigger and more secure in the backing, which is similar to this one. The lowest tension setting on my chair is still too high for her to lean back on my chair. This chair has a lower tension minimum and she can easily lean back freely, as well as keep enough tension to work without sagging. This chair is surprisingly outstanding! I’m sure my wife will destroy it quicker than the last one, but it’s so cheap that even if it only lasts 1-3 years, I’ll happily buy it ones or twice more 🤷🏽‍♀️. Maybe by then they’ll invent a comfortable titanium chair that’s affordable that she won’t destroy 😂.
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Reviewed in the United States on February 28, 2024
R
Verified Purchase
Rebecca Olmos
West Palm Beach, US
★★★★★ 3
Mid.
Color: Light Purple
The chair is fine, and reasonably easy to put together. However, I've been using it for a little over a year and a half, and it's already falling apart. Random parts of it have fallen off, which is fine because it was still functional. But now the most frustrating part is that the wheels continue to pop off, and while the first attempts to pop them back on worked, they will not stay on now. This is obviously greatly impacting the stability, and I will need to purchase a replacement soon. For this price and lack of long-term durability, I would not buy again.
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Reviewed in the United States on January 4, 2026
B
Verified Purchase
Bridgett Kelly Rode
Omaha, US
★★★★★ 5
Alexa messed me up, but the company of the chair made it right!
Color: Light Purple, Color: Light Purple
I wanted something small with arms that moved out of the way when I wanted to slide my chair all the way into my desk for more space. This fit the bill. I loved the color blue and I had that in my cart for a bit. One day Alexa mentioned that an item that was in my cart was on sale. It was the chair. I let it be ordered. When it came it didn't exactly look blue to me. But I kept putting it together thinking maybe it was the lighting in the room I was assembling it in. I brought it to my craft room and lo and behold it is light purple. I looked at my order and sure enough it said light purple. Boo! I looked in my saved cart and the chair was gone. So I accepted it for an error. The next day I checked to see if I could return it. Thought I could, till I read that I would have to pay to return it. So I wrote the seller to tell them to ignore the return. And that was that. Till a couple days later I get an email from the seller asking if I would like a full refund or a replacement at no cost. I was like heck yeah send me the blue one. I was thrilled and I wanted to thank the seller for doing this without the return of the other chair. But I could not find a place to do that now. So here is where I will thank you. Thank you so much for doing this for me. You guys are AWESOME!. I love the chair and I love the blue color. I find it very comfortable, lite, easy to adjust, the size works great for me, and for the price the quality fits my needs. I do hope it lasts me for quite some time. Thanks again!
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Reviewed in the United States on January 9, 2026

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