Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 22 - Jul 27
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse NETs ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a neutrophil extracellular trap network (NETs) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of neutrophil extracellular trap network (NETs) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Neutrophil extracellular trap network ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Neutrophil extracellular traps (NETs) are networks of extracellular fibers, primarily composed of neutrophil DNA, that bind to pathogens. Neutrophils are the immune system's first line of defense against infection. NETs enable neutrophils to kill extracellular pathogens while minimizing damage to host cells. Upon in vitro activation with the pharmacological agents phenylpropanol acetate (PMA), interleukin-8 (IL-8), or lipopolysaccharide (LPS), neutrophils release granules and chromatin, actively forming an extracellular matrix of fibers called NETs. NETs are produced in large quantities at sites of inflammation and deliver high concentrations of antimicrobial molecules locally, trapping and killing various pathogens, rapidly controlling infection and providing an antimicrobial immune response. NETs eliminate a variety of pathogens by locally delivering high concentrations of antimicrobial proteins. They can kill Gram-positive bacteria (such as group A Streptococcus, Staphylococcus aureus, and Streptococcus pneumoniae) and Gram-negative bacteria (such as Salmonella and Shigella), as well as fungi (such as Candida albicans). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 18 reviews
Sort
Product Reviews
★★★★★ 5
Works great
Style: 7-in-1 【2 HDMI】
The Anker 7-in-1 USB-C Hub (Dual HDMI) is an excellent docking solution for anyone looking to expand their laptop’s connectivity without carrying a bulky dock. Setup was extremely simple—just plug it into the USB-C port and everything works instantly.
The dual HDMI outputs are a major highlight, allowing easy connection to two monitors, which makes multitasking much more efficient. The 85W pass-through charging is also very convenient since it powers the laptop while using the hub, eliminating the need for multiple cables.
Build quality is exactly what you’d expect from Anker—solid, compact, and well-designed. The hub feels durable, doesn’t overheat, and the ports are well spaced so cables don’t interfere with each other.
Overall, it’s a reliable and powerful USB-C hub that’s perfect for work-from-home setups, office use, or travel. If you need dual monitors and multiple ports in a small device, this is a great choice.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 11, 2026
★★★★★ 5
Excellent Hub for Work Setup
Style: 7-in-1 【2 HDMI】
Very practical and efficient USB-C hub. Everything worked well right away and it made my workspace setup much easier and more organized.
The dual monitor support is excellent, the connections are stable, and the overall quality feels premium.
Perfect for work, multitasking and connecting multiple devices without complications.
Very happy with this purchase!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
★★★★★ 5
Perfect USB-C Hub for Dual Monitors, Engineering Work, and WFH
Style: 7-in-1 【2 HDMI】
This Anker 7-in-1 USB-C hub has been a great addition to my setup. I'm using it with my HP Victus gaming laptop to run dual external monitors, and it handles everything flawlessly—no lag or resolution issues, just clean and stable output.
I use it daily for engineering work and working from home, and it’s made my setup far more efficient. The 100W power delivery keeps my laptop charged while powering other devices, and the 10Gbps USB ports make transferring large project files fast and hassle-free.
It’s compact, well-built, and stays cool even during long work sessions. The design is smart, with well-spaced ports that help keep things organized.
Best of all, the price is very affordable for what you get—especially considering the dual-monitor support and power delivery. Excellent value for a high-quality, reliable hub.
If you're building a dual-monitor workstation for WFH and technical work, this hub is a solid, budget-friendly choice.
UPDATE: I've been using this dock for a while now and it is so great to have if you are wfh. My wife liked it so much i ordered one for her desk setup. Thank you Anker!
Joe
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2025
★★★★★ 1
Terrible for dual monitor!
Style: 7-in-1 【2 HDMI】
In 2026 1080p 60 on dual monitor is atrocious and not obvious or reasonable to expect unless you very carefully look at the description images. Returned. Unacceptable and ridiculous. They need to either stop selling this or make that limit much clearer. It’s 2026, and this should be able to handle 2 1440p monitors at 60hz minimum. Really it should handle 2 4K at 60 or 2 1440p at 120hz minimum. A cheap 3 year old dock a got can handle 1440p at 90hz on two monitors.
It’s ridiculous especially because it’s rated for 10 gbps. A person would reasonably think that means it’s good good for this.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 9, 2026
★★★★★ 4
Good
Style: 7-in-1 【2 HDMI】
Works great. Only complaint is that now my screens will shut on and off sometimes and I wonder if it’s the cord going out or an issue with my monitors.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 27, 2025
recommand products
The Ashton-Drake Galleries Disney Princess Timeless Tales Tots Figure Collection Issue #1: Princess Belle Handcrafted & Hand-Painted Resin Figurine with Fabric Accents 4-Inches
42.95
The Bradford Exchange Light of Hope Illuminated Ship-In-A-Bottle Sculpture Handcrafted Seascape Art with Illuminating Lighthouse Clear Glass Bottle and Compass Base by Thomas Kinkade 8.75" L x 5.5" H x 4.75" D
98.00
The Bradford Exchange A Bushel And A Peck For Great Granddaughter Featuring An Engraved Filigree & Scalloped Edging Handcrafted Wooden Music Box 6-inches
82.98
The Bradford Exchange Spirit of Destiny from Luminous Spirits Sculpture Collection Issue #2 Hand Painted Fully Sculptured with Feather Wings Illuminated Fantasy Sculpture 8.5-inches
74.98
10K Birthstone Oval Ring
150.00