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Description
Mouse TMX1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against thioredoxin-related transmembrane protein 1 (TMX1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of thioredoxin-related transmembrane protein 1 (TMX1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Thioredoxin-related transmembrane protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Thioredoxin-related transmembrane protein 1 (TMX1) is a protein encoded by the TMX1 gene. TMX1-associated disorders include ciliary dyskinesia, primary, and 6. Gene Ontology (GO) annotations associated with this gene include protein disulfide isomerase activity and disulfide oxidoreductase activity. An important homologue of this gene is TMX4. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.5 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 3
Not a happy customer
My Aussie ate the chew stick within 5min. Disappointed it didn't last longer. Won't buy again :(
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 4
Massive treats, no tummy rumbles, but a little pricey
I'm always a little tentative when ordering dog treats online, because you don't always know in advance where they're sourced from, or what weird processing might be done to them, or what weird additives might be thrown in. These are sourced in Mexico, and they seem to be straightforward in terms of ingredients. Phew!
The smell of these is not the best, but if you've fed your dog collagen treats before, I'm sure you know what you're getting into with that. The dogs loved them, for sure. These treats are MASSIVE, so if you have a small or medium sized dog, you might want to cut them into two or more pieces. For my 60ish pound dogs, they were very large but manageable, and the way their eyes lit up when I presented it to them, it was like Christmas. LOL
The only reason I'm docking a star is because these are not long-term chews, at least for my dogs. Yak chews will generally last for a couple of hours for them, which I break up over several days by taking the treat away after a while and giving it back to them the next day. These chews were gone within about 5-10 minutes. (Five minutes for my spazzy dog who acts like treats will disappear if they're not hoovered it into her gullet at breakneck speed, and ten minutes for the other who likes to taste his food on the way down.) At almost $6 a pop, these are pretty expensive for 5-10 minutes of enjoyment, so this isn't going to be a treat I buy for them on the regular.
There were no issues with tummy rumbles or nausea or diarrhea afterward, so that's good. And I like getting a little more collagen in these kids because they're both getting up there in age, so their hips and joints can use a little extra love. If you've got the extra money to spring for these, or if your dog paces him- or herself reasonably with dog treats, this could be a good choice for you.
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Reviewed in the United States on September 8, 2025
★★★★★ 5
My non-chewer loves these
He is not into chewing, tried many different ones.
But he’ll chomp down on theses, and they seem to be all good stuff
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Reviewed in the United States on December 17, 2025
★★★★★ 5
Dog loves them, no rawhide!
My dog is obsessed with these! We have a 70 pound pit mix who is a power chewer and these rolls last forever. He goes crazy for the peanut butter taste and it takes him a couple of days to really destroy one entirely. They are very effective for keeping him busy as the size is massive. I like giving him something so large because he then sets his mind to it for a long time. I like that they have a lot of collagen in them, I can't say I've noticed that his coat is a great deal shinier but it can't hurt! I also like that these are rawhide free. I would say these are a good value as they keep him busy for a good amount of time.
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Reviewed in the United States on January 29, 2026
★★★★★ 5
These are great my dog loved it
Well, I can say one thing I have shepherd. Wolf hybrids, and they are very particular about the stuff they eat. I can give them raw meat, and if it's old, they don't want it, so they're pretty finicky, I hooked them up with these. And they didn't hesitate to go at them. So I wait, I fed half, and then waited a couple of days, they had no negative response to it. That I'm aware of and so I gave him the other one and split it between them, and they don't stop until they're gone. So they pretty much sold themselves for me. If mine My dogs will eat em.They must be okay worth the money in my book.Made my babies happy should get some for yours my dogs are like super chooers.Okay, they have jaws that break bone.So to try to say that these are not good for heavy chewers, I i can't agree with that.Although they won't last very long with heavy chewers, they do great.The dogs love them we only smell was the smell of the product.Kind of a food smell to it.And as soon as I opened the package, the dogs had me surrounded like the sue did old custard, and they weren't letting me go until I shared straight up the male's a hundred plus pounds.And he's eyeballing me like, i'm gonna be lunch.If I don't give him a share, it was funny as hell a great product i think one of the things is the peanut butter.That's in them.My dogs love their peanut butter now.These are almost a foot long each stick, and they're about an inch and a half in diameter.Now they would last for a pretty good time for a normal chew type.DogRottweiler's pit bull saint bernard's high bread.Shepherds, et cetera, anything with a two thousand pound byte is going to tear these things up and have a great time.Doing itThey won't last as long as the dainty chewers and the the comfort chewers, but they'll do the job
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Reviewed in the United States on September 13, 2025
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