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Description
Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. |
| Experimental Procedure | https://univ.oss-cn-shanghai.aliyuncs.com/absin-china/images/experiment/20190514165940524.png |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 540, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.5 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
Great product
Color: Black
This is very sturdy and great quality. Its quite cheap for what your getting to it’s not cheap thin like plastic it feels thick and you could put a LITTLE weight on it it’s stable and protects your privacy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
★★★★★ 5
Excellent privacy shade.
Color: Coal Black
This delivered on exactly what it promises. So happy with the quality for the value. The shades are a fabric, nylon like, that are attached with a pocket on one end and velcro that adheres around the bar on the other.
This was incredibly easy and straightforward to put together. Every piece was clearly labeled, coordinated with the instruction sheet, and fit perfectly. Honestly, I wasn’t planning to leave a review but I was so impressed by how clearly labeled and well explained the instructions were that I felt compelled to note it. Especially for anyone who doesn’t like putting things together, or is used to subpar instructions, this is leagues above the rest. It’s such a simple build anyway, but after buying so many things that barely fit as they should, this stands out.
It is lightweight and easy to move. Offers privacy when opened, then can be folded out of the way. It looks exactly as pictured. No complaints, it’s a solid item and worth the price.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Decent quality for the price
Color: Grey
Decent quality for the price. Other privacy screens were extremely out of my budget, but this looks and works just fine- I just needed something simple to place behind my couch to hide some clutter. It took me about a half hour to assemble, but it was easy enough.
It might be due to my carpeting, but I don't trust that the screen would be stable enough to stand freely without falling. Thankfully my
plan all along was to wedge the screen between the couch and a box of clutter anyway, so for me it works as l'd planned!
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Reviewed in the United States on April 10, 2026
★★★★★ 4
Overpriced but effective.
Color: Cream
I happened to get lucky and get a warehouse deal at an additional 30% discount. Even at $25, it feels a little overpriced. But it was easy to assemble and serves its purpose. If you line the Velcro up exactly, the panels sag, if you stretch past even, but where it still grips, they look pretty ok. It is very light and does fold very compact for easy storage.
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Reviewed in the United States on April 3, 2026
★★★★★ 5
Great price for a great product
Color: Coal Black
Excellent quality and great price to boot. Easy to assemble and folds up easily to be stored. We are using it as a privacy screen when we have company that needs to sleep on the couch and also to block off a hallway leading to a guest suite. It looks great and is as described in the ad
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Reviewed in the United States on December 16, 2025
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