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Description
mIHC Dye (TSA620)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 620, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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Product Reviews
★★★★★ 5
DO BUY!
Size: 30 Count (Pack of 4)
These facial wipes are perfect for removing makeup or in the morning & all for a super price!
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Reviewed in the United States on May 12, 2026
★★★★★ 4
Thorough and Gentle
Size: 30 Count (Pack of 4)
Not super moist, but they are soft and do their job. I only use wipes if I’m in a pinch to get my makeup off and don’t have my usual face wash. I’m very satisfied with each type of wipe included in the bundle.
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Reviewed in the United States on April 16, 2025
★★★★★ 5
Great product
Size: 30 Count (Pack of 4)
Only just received, so I haven't had the chance to test them. So far they are compatible with my skin and easy to use.
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Reviewed in the United States on April 27, 2026
★★★★★ 3
Buyer beware. For your sake read my review please.
Size: 30 Count (Pack of 4)
I loved the smell which can be kind off setting especially if unscented. However the love kind of stopped there. The size of the wipe it self was not much than one of those wipes you get at the BBQ store with dinner. I do not wear foundation or heavy makeup however I had to use 3 wipes each time before I felt my skin was clean. I was excited to have the different issues of my skin dealt with as far as anti-aging, pore refining etc but there was not a single bit of difference between the wipes other than the scent which again was very spa like and pleasant. Easy to like. Small wipe. Need several. By the time you add up the added cost of the extra towels each day and I could have purchased a much higher quality product. I don't forget to look at reviews very often, but this time they sounded so positive and I went ahead and purchased. Sorry I ever did but glad I didn't order two or three due to the sale on them.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
Great for sensitive skin!
Size: 30 Count (Pack of 4)
Works really well. No burn. I have very sensitive skin and these have been great. Not overpowering.
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Reviewed in the United States on April 7, 2026