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Description
Mouse CTTN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with cortactin (CTTN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of cortactin (CTTN) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Cortactin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cortactin (derived from "cortical actin-binding protein") is a monomeric protein located in the cytoplasm that is activated by external stimuli to promote polymerization and rearrangement of the actin cytoskeleton, particularly at the cell periphery. It is present in all cell types. Upon activation, it recruits the Arp2/3 complex to existing actin filaments, promoting and stabilizing actin branching nuclei. Cortactin is important for promoting lamellae formation, invasive cell formation, cell migration, and endocytosis. Diseases associated with CTTN include sigmoid sinus adenocarcinoma and Wiskott-Aldrich syndrome. Pathways associated with CTTN include developmental Gap-Rob signaling and Syndecan-3-mediated signaling events. Gene ontology annotations associated with this gene include profilin binding. An important homologue of this gene is HCLS1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
Excelente
Excelente
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 12, 2026
★★★★★ 5
Great way to control TV and radio in one room from an easy chair in another room!
I help a 96 year old blinded vet with tech support -- and as his health worsens, needed to make his TV, his radio, and the audio switch that selects what audio should go into his hearing aids via Bluetooth work from his easy chair in his living room. All the equipment is in his bedroom. This IR repeater system installed quickly and easily and works perfectly. Now he can summon ball games, news, or whatever he wants without fear of getting up and falling down. A perfect solution!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
Magic!!!
I am no tech expert but these little guys are magic! We had just put in a wall of built-ins and desperately wanted to hide our cable box behind a cabinet, but with it being infrared this would not work without these little repeaters! First off I just have to say that the Sewell customer service is off the charts. You actually get to speak to a real person right away, no annoying menu after menu trying to get help. They helped us so much when we hit a couple snags. Here are a couple things that I figured out that I hope will help others. First you can hide both of these little discs, they both come with tiny little eyes that can attach to your tv and cable box or whatever you are trying to use them for, and they are nearly invisible. Second, don’t get nervous when you plug them into the power outlet and the lights don’t come on, this is ok and they aren’t supposed to stay on, they kind of blink here and there, and only when you use your remote. Third, we have an Altice One cable box (not the mini, just a regular cable box) and it was impossible to find the eye to attach the emitter’s eye (Side note: we had to put the emitter disc on top of the cable box because we couldn’t fit the emitter disc pointing in front of cable box. The directions give you both options though and you can basically put it anywhere as long as the eyes attach). The phone trick did not work on this particular cable box. But after trying about a dozen different spots on the front of the box we finally got it to work by putting the eye right on where the digital clock numbers are. I put it basically right on top of the colon (:). Cable vision was no help with this so I hope this can save others from our frustration. After we found the eye everything came together and worked perfectly! No more do we need to keep the cabinet open to change channels… it’s a miracle!!! Hope these little guys work forever.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 22, 2022
★★★★★ 4
Good product, expensive price, poor adhesive
Premium packaging for a premium device. Useful for IR remote signal delivery with extra functions on the side. Price though is luxury for it’s use. Used this for a Logitech Harmony hub control of an non-smart electric fireplace. Setup is a breeze but only if you’re tech savvy. Highly recommend to watch the YouTube video for information on all it’s application. There a many other cheaper devices similar to this available on Amazon that I could have used for the same application. If you have a need for multiple control within the same vicinity of other devices, this may be more useful for you. However, if you plan on utilizing an IR repeater/extender for only a single device and would like to save some money, don’t buy this one. This device works quite well though. Have had no issues achieving the effect needed. Only four stars due to the poor adhesive on the emitter attachment. As stated earlier, I used this on an electric fireplace with a glass surface. I used the attachable emitter to the front glass sensor where the IR receiver was located on the fireplace. After only one day, the emitter attachment fell off even though I made sure the glass was thoroughly cleaned prior to adhering it. Otherwise a good product.
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Reviewed in the United States on November 8, 2019
★★★★★ 5
Denon avr ir remote port works great with sewell products
I bought a new entertainment center with glass doors and wasn't getting good signal range with the door closed. Plus i couldnt get a good of line site, since this cabinet was shorter than the orginal, and the location for my avr was further to the left than before and now my line of sight to the receiver was blocked from other parts of the room due to high couch back.
I tried a couple different cheap $10 devices that used stick on transmitters/receiver buttons but they were clumsy and didn't provide me a total solution for my room layout.
Works fantastic with a denon x1800 receiver (and many other models). It works directly with the denon's ir remote port in the back of the avr port. The solution is actually quite simple.
Plug a stereo 3.5 mm male to male plug in the denon ir port in the back of the avr and and the other end to the orange emitter box(puck). The pics are about the size of an oreo double stuff cookie Tuck the orange box/puck away whereever and plug the power in. The box doesn't need to seen or be pointed towards anything. It's location is only limited by the length of your stereo cable and access to AC power
Put the blue box puck anywhere in the room where you have general line of sight to it from where you will be using your remote, and plug power in. In my case, its sitting on top of one of my tower speakers. From there it clears the back of the couch and can see from the family room, eat-in kitchen area, and the kitchen beyond.
Done.
I didn't need or use any of the included emitter or transmitter cables, though if you dont have a denon, or other brand avr with an ir port to plug into , or also want to hide the blue puck ( in which case attach the included sensor cable), you'll need them. But for denon users with an ir port, you just use the two pucks and their usb power adapters. Simple.
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Reviewed in the United States on April 17, 2026
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