Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 22 - Jul 27
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human FBN1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a fibrillin 1 (FBN1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the fibrillin 1 (FBN1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Fibrillin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Fibrillin-1 (FBN1) is a protein encoded by the FBN1 gene located on chromosome 15. It is a 230 kb gene with 65 coding exons that encodes a 2,871-amino acid proprotein named profibrillin. This proprotein is cleaved near its C-terminus by the furanyl invertase protein to yield fibrillin-1, a member of the fibrin family, and asprosin, a 140-amino acid protein hormone. It is a large extracellular matrix glycoprotein that serves as a structural component of 10-12 nanometer calcium-binding microfibrils. These microfibrils provide force-bearing structural support in elastic and inelastic connective tissues throughout the body. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 8 reviews
Sort
Product Reviews
★★★★★ 4
An Excellent Continuation For The New Dynamic Duo
I really loved Batman and Robin Volume 1. This arc is my favorite part of the Morrison Batman run, even though I really struggle with and don't enjoy everything that comes before it. But it's worth it for this duo of Dick Grayson and Damian Wayne.
Volume 2 gets off to a slow start with a few issues about a deranged Batman resurrected by a Lazarus pit. Readers who haven't read Morrison's other work, including Final Crisis, will be confused by what occurs here. But the gist is that Dick and Damian learn what the readers already knew, that the corpse they thought was their father wasn't his (instead a failed clone from a Darkseid experiment), and that their father is out there somewhere. The art in these initial issues is also a bit rough and lower in quality, which is the only reason I brought this down to 4 stars.
The second story in the remainder of the volume though, that pits Damian against Dick and tests Damian's loyalties is a great continuation of the Batman and Robin story and might be one of the best parts of the series. Damian may be rude and sociopathic, but this arc gets to let him shine a bit and show that he really does care about his big "brother" Dick Grayson, and about his father's legacy. As a bonus, there is a running mystery about the duo's new ally Oberon Sexton, which I thoroughly enjoyed. I won't spoil the ending, but it's a truly fun reveal that makes you rethink Sexton's role in the adventure in an interesting way.
Again, this is a great story for Batman/Dick Grayson/Damian Wayne fans that I strongly recommend.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 7, 2019
★★★★★ 5
Not Perfect, But Loved It Anyway!
Format: Hardcover
The cover and title of this collection are slightly misleading, but it's still a fantastic read. Robin isn't willingly trying to kill Batman, however, I'll let you find out what's really happening yourself. I love the new Batman and Robin team of Dick Grayson and Damian Wayne. It's a fun change of pace to have a more lighthearted Batman, and a bratty and brooding Robin. This is the second collection of the new Batman and Robin series, but I still enjoyed it without having previously read all of the first volume.
The Highlights: Batwoman, a zombie Batman, Talia al Guhl, Slade Wilson, the mysterious Mr. Sexton, the history of the Wayne family, and the beginning of a Joker story line. The new flying Batmobile is awesome. I love Grant Morrison's writing, and here he writes a fun and interesting Batman adventure.
Things to Consider:There are two different artists in this volume. The first half is decent, and the second half is more unique with some great pen and ink work. I enjoyed the second part a lot, but it's not quite the classic super hero style art you might expect.
This may not be a groundbreaking story, but this series is great. So if you're interested in Batman and Robin, you won't regret checking this out.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2011
★★★★★ 5
Wow! Great Stuff!
Format: Hardcover
Before I read this book, I had just read the other recent Batman trade, "Life After Death" by Tony S. Daniel, and I was seriously disappointed. I really struggled to get through it.
So the next night, I started to read this one, Batman vs Robin, and I couldn't put it down - I almost read the whole book in one sitting (if it wasn't late at night, I probably would have finished it). It just confirms, once again, what a great writer Grant Morrison is.
In this trade, you get two main story arcs (with a nice "cameo" by Batwoman in one story) and the search for Batman really starts to develop, here.
I enjoyed Volume 1, but IMO, this one is far superior.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 18, 2010
★★★★★ 5
It gets AWESOME. I highly recommend this
First volume is kind of a drag, but it gives backstory and character development for this volume 2. It gets AWESOME. I highly recommend this, it has mystery, action, and great art. Fun read and you MUST read the first volume.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 14, 2017
★★★★★ 4
Batman & Robin: Batman vs Robin
Format: Hardcover
First things first... This book will only make sense to you ONLY if you've been following Grant Morrison's complete run of Batman (including Final Crisis). If you haven't, most likely you won't like it or you'll get half the excitement this baby carries.
I used to think I didn't like Frank Quiely's art, but after the psychedelic style he put on the first volume, I found myself missing him in this particular book.
The art in the first 3 chapters (The Blackest Knight) is terrible. So simple and flat. The writing is good, but the art took whatever excitement for me.
On the other hand, the art on the next chapters is quite better, and together with the story I end up wanting more.
I have to give it to Grant Morrison. If they give him time and creativity control, he can make things happen (although it would be better if Dan Didio wouldn't make him re-write the end of Final Crisis).
There's only 2 things I don't like about all this (besides the art on the first 3 issues): FIRST - I don't understand WHY Dick Grayson have to use electric knuckles every time he fights. SECOND: Seems to be now everybody knows Batman's secret identity.
I liked the book as a whole, and I can't wait to check it all out now that lots of the beans has been spilled.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 19, 2011