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Description
Human TNF-α ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human TNF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa. TNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.5 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
As promised
Format: Paperback
Accurate product, no damage or marking noticed
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 6, 2025
★★★★★ 5
Good book!
Format: Paperback
Very helpful book for pathophysiology. The CD not only gives you answers to the case studies but gives you clear details of the clinical Pathophysiology of a particular disease. Definitely recommend it to students going for their Master's degree!
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Reviewed in the United States on February 19, 2015
★★★★★ 3
No Answers Provided to Case Studies
Format: Paperback
The cases are helpful; however, realize that there are NO answers whatsoever to the questions presented in the cases--not a huge problem I suppose, b/c you can google everything, but certainly an annoyance. (esp. when there are multiple possible answers. The author states in the foreword that some of the cases are real; in which case, why not provide the justification presented in the patient's chart as to why that patient takes a particular drug, esp. considering that you may take a drug for its side effects rather than its therapeutic effect. . .anyhow. . )
I think if the author provided at least SOME insight/guidance to the cases that it would be much more helpful, but until then, I'm skeptical as to why others gave this book 5 stars.
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Reviewed in the United States on July 23, 2016
★★★★★ 5
... for a Patho class and thought it was a great resource. My group had hypertension and it was ...
Format: Paperback
Used this for a Patho class and thought it was a great resource. My group had hypertension and it was really good about giving an overview of the disease without overwhelming you with information. Some of the studies were obvious as to why the disorder occurred but in those cases it helped link body systems and show interactions.
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Reviewed in the United States on June 19, 2015
★★★★★ 5
Brand new
Format: Paperback
Brand new, fast delivery!
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Reviewed in the United States on April 8, 2024