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Description
mIHC Dye (TSA700)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 700, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.3 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
We really like the idea and ease
Pattern Name: Detachable high wall, Pattern Name: Detachable high wall
Right out of the box, it comes with pee pads included, which is a thoughtful touch and lets you start using it immediately without needing to buy extras first. The design is simple yet highly effective, especially for dogs who tend to lift their leg or accidentally aim a bit higher than expected.
The removable high wall guard is one of the standout features. It does an excellent job preventing urine from splashing outside the pad area and keeps surrounding floors, walls, and furniture clean. Because the splash guard is detachable, it can be removed easily when needed, making the entire unit extremely user-friendly when it comes to cleaning and maintenance.
At 23" x 23", the size is generous without being bulky. It offers plenty of space for small to medium dogs to move around comfortably, and it gives puppies a larger target area as they learn proper potty habits. The frame holds pads securely in place so they don’t bunch up or slide around, even if a dog circles or shifts while using it.
The durable plastic construction is another strong point. It feels sturdy, not flimsy, and holds up well to daily use. Cleaning the plastic is simple—usually just a quick rinse and wipe-down is enough to keep it looking and smelling fresh. Changing out the pee pads is also quick and hassle-free, making upkeep easy even on busy days.
It keeps messes contained, simplifies pad changes, and offers a durable, spacious setup that both dogs and owners can appreciate. It's a great choice for indoor training or long-term use.
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Reviewed in the United States on November 25, 2025
★★★★★ 5
Works Great & Very Practical for Indoor Potty Training
Pattern Name: Detachable high wall
I really love this pee pad tray. We have a similar one from another retailer that has worked well, and I wanted a second tray for our multi-story home. My puppy had no trouble using this right away since he’s already trained to use a pad when we’re not able to take him out.
The design keeps his paws dry, which I appreciate, and it even came with wee pads included. The splash guard is a nice touch for male dogs my puppy isn’t lifting his leg yet, but it will definitely be useful later on.
Just a note: if your dog tends to have softer stools, cleaning the small holes in the tray might take a little extra effort. For us, that hasn’t been an issue, and the tray is easy to rinse off when needed.
Overall, this is a much cleaner and more reliable option compared to indoor grass pads or loose training pads that some puppies chew up. I’m really happy with it and would recommend it.
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Reviewed in the United States on December 4, 2025
★★★★★ 5
Reliable, No‑Mess Pads That Actually Work
Size: X-Large, Style: 40 Count
I’ve tried a bunch of different puppy pads, but these Amazon Basics ones are easily the most reliable. The 5‑layer design really does make a difference—no leaking, no soggy spots, and the quick‑dry top layer keeps my puppy from tracking wet footprints around the house. They absorb way more than I expected, and the plastic backing has never let anything seep through to the floor.
The size is perfect for daily training, and the pads stay flat instead of bunching up. My puppy took to them right away, and cleanup is super easy. For the price and the quality, these are honestly the best value pads I’ve found. Highly recommend for puppies, small dogs, or even senior dogs who need a little extra help.
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Reviewed in the United States on March 29, 2026
★★★★★ 5
No leak pads
Size: Regular, Style: 50 Count
No leak pads, good quality, thickness is good and decent price.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Price and product doing its job well
Size: Regular, Style: 50 Count
Perfect size and absorbtion is good.
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Reviewed in the United States on May 18, 2026