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Description
Rat CASP12 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a caspase 12 (CASP12) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of caspase 12 (CASP12) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Caspase 12 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Caspase 12 (CASP12) is a protein encoded by the CASP12 gene. This protein belongs to a family of enzymes known as caspases, which cleave their substrates at the C-terminal aspartate residue. It is closely related to caspase 1 and other members of the caspase family, known as inflammatory caspases, which process and activate inflammatory cytokines such as interleukin 1 and interleukin 18. This gene is polymorphic, producing either the full-length caspase protein (Csp12L) or an inactive truncated form (Csp12S). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
Sway Alert!! This is a beautiful suite!
Color: Beige, Size: 5, Color: Beige, Size: 5
This suit fit great my son loved it. The quality is good. My son is five about 40 inches tall around 40 pounds. He’s very slim but the length was great. The suit jacket fit well. The pants waist were a little big, but the adjustable strap helped with that, but the fit overall was great . I would purchase a suit again in a different color.
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Reviewed in the United States on April 12, 2026
★★★★★ 5
Great Looking Suit with Lightweight Material
Color: Light Grey, Size: 7
I got this for a beach wedding and it worked out really well. It looked nice without feeling too formal, which was perfect for the setting.
The material is linen and it feels lightweight and breathable. It kept my kid comfortable in the heat and didn’t feel stiff or heavy like some dress suits do.
The appearance is sharp. The double breasted style makes it look more dressed up, but the linen material keeps it relaxed and appropriate for outdoor events.
The fit was true to size. It sat well without looking baggy or tight, and everything stayed in place throughout the event.
Quality feels good for the price. The fabric and stitching don’t feel cheap, and it held up well after a full day of wear.
Suitability is great for things like weddings, parties, or photos. It works especially well for warmer weather because of the material.
For value, I think it’s worth it. You’re getting a nice looking suit with comfortable material that actually works in real situations.
Overall, great fit, breathable material, and a clean look that works really well for events.
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Reviewed in the United States on March 28, 2026
★★★★★ 5
Very nice linen suit for boys. Well worth the piece
Color: Beige, Size: 12
This JPF Double Breasted Linen Suit for Boys is the cutest thing I've ever seen! I got itt for my 8 year old and he loves it! He tried it on at soon as we received it and wore it all evening. Lol. He looks so grown up! He's about 4'3", about 75lbs. I ordered a size 12 and it fits him the way I'd expect it to. A bit big but he has room to grow into it. It is a 2 piece suit, with pants and blazer only. It's a summer suit, it's meant to have a more laid back, casual style. It is so nice! The style and color are identical to the photo. I got the beige color, that's the color my kid picked. I do worry a bit about staining, considering an 8 year old boy will be wearing it, but hopefully any stains will be easy to remove. The quality is excellent and the price isn't bad either. I highly recommend this suit
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Reviewed in the United States on March 18, 2026
★★★★★ 5
A charming summer suit
Color: Light Grey, Size: 6, Color: Light Grey, Size: 6
This suit fit true to size. The light grey wasn’t exactly the same as advertised (see photo), but I still really like it. My son felt snazzy in this suit, and he said it was comfortable to wear.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Nice suit for the price!
Color: Light Grey, Size: 12, Color: Light Grey, Size: 12
This really is a very nice boys suit, especially for the price they are asking. My kids don't wear suits too often, maybe once or twice a year for special occasions so I don't need really expensive suits for them. The material is actually really nice on this. The color looks great and the pieces are sewn nicely. The adjustable waist band is really nice on the pants because my kids are on the slimmer side and this helps with fitting. You can also wear a belt with these if needed. I washed on delicate cycle and dried on delicate cycle a little, then finished by air drying. They washed and dried very nicely with minimal wrinkling. The front pockets are real and useable on these, which isn't always the case with boys suits. Overall would 100% recommend
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Reviewed in the United States on March 12, 2026