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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.3 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 3
Not addidas
Color: B-red Stripe, Size: X-Large
Material is a bit on the rough side. Expected a more moisture resistant
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Reviewed in the United States on May 24, 2026
★★★★★ 5
ThermoMaven Does All the Work and Eliminates All the Temp Worries
I made this purchase as a cost-effective way to monitor the meat temperature while cooking without any wires. I have used this in my smoker grill, traditional gas grill, charcoal grill, oven and rotisserie air fryer. I tested the reading for accuracy with other temp measuring devices and find that it has been spot on in every method. It is so easy to use that you push it into the meat to the designated line and it reads the ambient, (surrounding), temp as well as the current meat temp. The display is easy to read and has a backlight when cooking outdoors in dim lighting. It is magnetic so it keeps the controller in place. When finished easily clean the probe with dish detergent and a wipe. I will be purchasing the 4-probe version since I do a lot of smoker grill cooks so I can measure more than one zone or different foods at the same time.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Works great! For basic temperature tracking it's a useful tool.
After years of relying on a combination of my pellet grill's temperature probe and my trusty instant read thermometer, I decided to get into the 21st century and invest in a wireless probe. Not wanting to spend a bunch of money, I saw that this was on sale and I figured that if it didn't work I wouldn't be out too much money.
As I write this, I'm using it. It arrived last night and I went through the setup process. It was SO easy to set up and the app is nice and simple to use. I started my pork butt cook at 7am this morning and the probe has worked flawlessly! I compared the temperature readings to my instant read and they're within a degree of each other, so I know that what it's registering is accurate.
To be honest, this isn't a huge game-changer for me as I've been smoking meat for years and have a process down. But I do like the fact that I can monitor my temperature remotely. I think where it will be the most impactful is in overnight cooks. I won't have to go out to my smoker I'm the middle of the night to check my temps. I can do that from the comfort of my bed!
Though I've only used it once so far, I'm very satisfied with it. It seems to be rugged and made for the long haul. I'm looking forward to future cooks!
Update: I've now done several cooks with the thermometer. My experience with it has been mostly positive. For tracking internal temperature, this probe is very accurate and right in line with my instant read thermometer. My only nit is that the ambient temperature sensor is a little finicky. It is slow to update, and I usually must wait 20-30 minutes for it to report the ambient temperature correctly. Not only that, you have to make sure that the probe depth line is right at the surface of the meat. I found that the margin for error is really slim with this. But other than that, it works great, and the app has made it so easy to track the cooking rate. My Thanksgiving turkey was spot-on this year and the probe helped immensely!
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Reviewed in the United States on August 21, 2025
★★★★★ 5
Best Tool I've Purchased In A Long Time!
Best tool I've purchased in a long time! This magnetic wireless meat thermometer is the perfect size for brisket, pork shoulders, roasts, and even steaks. Connected well with my iPhone, and looks like I have a professional thermometer. Slim and easy to use, the LED screen is easy to read and keeps me informed of my temps with audible beeps. The probe is easy to clean, and even after a long cook, it doesn't require much scrubbing. Durable and well worth the money.
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Reviewed in the United States on April 21, 2026
★★★★★ 5
Good Thermometer with a Great App
The user experience with this wireless thermometer is much different than with the Chef IQ ones. I had a Chef IQ for 2 years until my husband put it through the garbage disposal. The software sucked, but everything that did wrong, the Thermomaven does right. I don't even have to open the app bc as soon as I turn it the thermometer on, the temp pops up on my iphone as well as on the Thermomaven base - freeing up my phone. The screen is easy to read and is backlit.
My only complaint is that the probe is too big. You just can't use this thing with burgers or thin chicken breasts. I also got the Thermomaven instant read thermometer which will work in those cases, and it's great as well and thin enough for anything. These people really make great products. I'm impressed.
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Reviewed in the United States on April 17, 2026