SKU: 61188531430

Biotinylated Siglec-4a His&Avi Tag Protein, Human

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Description

Biotinylated Siglec-4a His&Avi Tag Protein, HumanProduct Specification Species Human Synonyms MAG, Siglec 4a, GMA, S MAG Accession P20916 1 Amino Acid Sequence Gly20 Pro516, with C terminal 10*His&Avi tag

Product Specification


Species Human
Synonyms MAG, Siglec-4a, GMA, S-MAG
Accession P20916-1
Amino Acid Sequence Gly20-Pro516, with C-terminal 10*His&Avi tag GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSNVSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPAVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGPGGGSGGGSHHHHHHHHHHGLNDIFEAQKIEWHE
Expression System HEK293
Molecular Weight 80-95kDa
Purity >95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1、Läubli H. et al. (2020) Sialic acid–binding immunoglobulin-like lectins (Siglecs) detect self-associated molecular patterns to regulate immune responses, Cellular and Molecular Life Sciences.77: 593-605.

Background

Sialic acid-binding immunoglobulin-like lectins (Siglecs) are cell surface receptors of the I-type lectin family that are expressed on innate immune cells and bind to sialylated and non-sialylated ligands. Myelin-Associated Glycoprotein (MAG), also known as Siglec-4a, is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. It is selectively expressed by myelinating cells including Schwann cells in the peripheral nervous system (PNS) and oligodendrocytes in the central nervous system (CNS). It is encoded by a single gene that is conserved among vertebrates. It is composed of an extracellular segment containing five Ig-like domains, a single transmembrane segment, and a cytoplasmic domain. Mature MAG exists as two isoforms, termed S-MAG (short) and L-MAG (long), due to alternative splicing of the cytoplasmic domain.
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Exchange/Return Notes
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SKU: 61188531430

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