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Description
mIHC Dye (TSA770) Two-step MethodProduct Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.6 ★★★★★
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Product Reviews
★★★★★ 2
too large and hard
This product is way too large for small dogs.
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Reviewed in the United States on April 10, 2026
★★★★★ 5
Ideal for small dogs
Good product for my small dog. She really loves it!
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Reviewed in the United States on April 16, 2026
★★★★★ 4
Good, But Not Suitable for Aggressive Chewers.
I recently ordered the Chewzzle TPR Dog Chew Bones (3-pack) and overall, they’re a good option for light to moderate chewers. The colors are bright and fun, and the textured surface seems to help with gentle teeth cleaning, which is a nice bonus. The material feels flexible yet durable enough for casual chewing sessions. My dog was immediately interested in them and got to chewing.
That said, these are definitely not suitable for aggressive chewers like mine. He had ripped and bit off pieces in a matter of mins. If your dog tends to tear through toys quickly or has a strong bite, these likely won’t hold up for long. They’re better suited for small to medium dogs that enjoy softer chew toys rather than heavy-duty ones.
For the price, it’s a fair value as long as you’re buying with the right expectations. Great for light chewers, but not the best choice if you need something truly tough or long-lasting.
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Reviewed in the United States on March 24, 2026
★★★★★ 5
Great set! 6 weeks in and we still use everything in this pack daily
Got this snuggle puppy set for my 10wo Goldendoodle. I gave it to her after she’d been with me (and away from her litter) for about 2 weeks, so she actually didn’t like the heartbeat, but she LOVED the pup, which was bigger than her when I got it. One particularly cold night, I stuffed glove warmers in the heart pocket for extra warmth (I don’t like to run the heat too much as it affects my sinuses). She snuggled right up to it. The shape and softness makes it her favorite sleeping companion, no matter what position she’s sleeping in. She’s now 16w and still loves this puppy, even though she’s much bigger than it now. This puppy is holding up to her mouthing/teething stage and I’ve found it easy to keep clean.
She loved the wabble ball stuffed with a little kibble, but quickly mastered it.
The lick mats have been a lifesaver and are used daily in this house. Love that I have a spatula just for her and they’re pretty easy to clean. This is an excellent value for what you get!
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Reviewed in the United States on February 19, 2026
★★★★★ 5
Puppy settled in quite well with it!
This was a wonderful product. We got it for our puppy and it still stays in kennel in my bedroom, the door of it open at all times so he can come and go as he pleases- I no longer leave the heart in it but he still loves it and carries it through the house, dragging it! I would very much recommend it
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Reviewed in the United States on May 5, 2026