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Description
Mouse IL-1β ELISA KitProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse IL 1 antibody was precoated on a high affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL 1 present in the sample combined with the solid phase antibody and the detection antibody to form immune complexes. After
Product Specification
| Theory | Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-1β antibody was precoated on a high-affinity microplate. The standard substance, the test sample and the biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-1β present in the sample combined with the solid-phase antibody and the detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm). | |||||||||||||||||||||||||||
| Composition |
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| Background | The interleukin-1 (IL-1) protein family contains the classical members IL-1α, Il-1β and IL-1ra, as well as IL-18, IL-33 and IL-1F5-10. IL-1α and IL-1β bind to the same cell surface receptor and share biological functions. "With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, unstimulated cells from healthy mice do not produce IL-1." "However, in response to inflammatory agents, infections, or microbial endotoxins, IL-1 is produced in large quantities by macrophages and a variety of other cells." IL-1β plays a key role in the physiological processes of immune response and inflammation, bone remodeling, fever, glucose metabolism, growth hormone /IGF-1. "Abnormal or sustained production of IL-1 has been associated with a range of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury, and aging-related diseases." IL-1α and IL-1β have structurally related peptides that share approximately 25% identity at the amino acid level. They were synthesized as the size of 31 kDa precursor, and then cracking into mature protein, molecular weight is about 17.5 kDa. Caspase 1 / ICEI cracking IL - 1 beta precursor is a key step in the inflammatory response. Neither IL-1α nor IL-1β contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through nonclassical pathways. Part of raw IL - 1 alpha can appear on the cell membrane, and may have biological activities. Unlike precursor IL - 1 alpha, beta IL - 1 precursor compared with mature body almost do not have biological activities. Both unprocessed and mature IL-1β can be exported from the cell. IL - 1 alpha and beta IL - 1 with IL - 1 ra combination immunoglobulin superfamily receptors play a role. "Cells expressing the 80-kda transmembrane type I receptor (IL-1RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid lining cells, chondrocytes, and hepatocytes." "Cells expressing the 68kDa transmembrane type II receptor (IL-1RII) include B cells, neutrophils, and bone marrow cells." "The two IL-1 receptors share approximately 28% identity in their extracellular domains, but a significant difference is found in their intracellular domains: the type II receptor has an intracellular domain of only 29 amino acids, whereas the type I receptor has an intracellular domain of 217 amino acids." IL-1RII does not produce a signal for IL-1 and may act as a decoy receptor to attenuate IL-1 function. IL-1 receptor accessory protein (IL-1RAcP) is associated with IL-1RI and required for IL-1RI signal transduction. IL-1ra is a secretory molecule that acts as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1RII can be detected in human plasma, joint synovial fluid, and conditioned media of a variety of human cell lines. In addition, IL-1binding proteins similar to soluble IL-1RII are capable of being encoded by cowpox and vaccinia virus. |
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| General Notes | 1. Please use the kit within the validity period. 2. Components of different kits and different batch kits should not be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1×) is used. "In addition to dilution, cell culture medium may be used for other intermediate dilutions." 4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Unopened kit, 2-8° C Storage | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.4 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
Neutrogena clear face loción de protección para el sol
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
Neutrogena Clear Face Sun Protection Lotion is one of the most popular options for the face, especially if you have oily or acne-prone skin.
General opinion: it is a very good sunscreen for daily use, light and designed not to cause outbreaks.
The positive:
* It is oil-free and non-comedogenic, that is, it does not usually cover pores
* It has a light texture and "matte" type, it does not leave the skin greasy
* Absorbs quickly and can be used under makeup
* Offers good SPF 50 protection against UVA/UVB rays
* Many people with acne skin say that it does not cause pimples
The not so good:
* On very oily skin it can be a little shiny over the hours
* It is not super moisturizing (if you have dry skin, it may feel a little "light")
* Some people may be slightly irritated if they have very sensitive skin
Final opinion:
It is a highly recommended sunscreen if you have combination or oily skin and want something that does not cause acne. It is practical, cheap and reliable for day to day, but it is not the most "perfect" if you are looking for a totally dry finish or super hydration.
If you want, tell me your skin type (dry, oily or mixed) and I'll tell you if it's the best option for you or if there's an even better one.
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Reviewed in the United States on May 16, 2026
★★★★★ 5
Matte, weightless, mildly drying
Size: 3 Fl Oz (Pack of 1), Style: SPF 50, Size: 3 Fl Oz (Pack of 1), Style: SPF 50
Very good sunscreen. It is very similar to the Neutrogena Ultra Sheer sunscreen but with less/minimal fragrance. It absorbs immediately and feels matte and invisible. The only slight downside is that the silica in the sunscreen is actually so good at absorbing oil that it is mildly drying. This is great for people with oily skin, but people with acne like myself often use retinoids that are already very drying, and this can magnify the dryness. I often follow this sunscreen with some moisturizer to counteract the drying effect.
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Reviewed in the United States on May 2, 2026
★★★★★ 5
No white cast.
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
Doesn't leave a white cast on your face as long as you rub it in enough. It also doesn't break me out. Good product.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Great - effective sunscreen that isn't oily
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
Works well, doesn't make your skin oily, almost disappears when you put it on. Made for faces but of course works anywhere else as well.
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Reviewed in the United States on May 8, 2026
★★★★★ 4
It is a good sunscreen, but if your skin is very oily, think again.
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
I have tried many, MANY sunscreens, and this one is in my top 10, but not at the very top. Why not? Well, what I like about it is that you get a good amount for an excellent price. It's lightweight, but not extremely so, and this size can easily last you 3 months without any problem. However, while it's made for oily skin, it's not that light. After just a few hours of application, it can leave your skin feeling greasy, especially if you sweat easily. It doesn't leave a white cast, but it does leave a slight, shiny finish. Reapplying isn't very comfortable. If you use it just once a day, it's fine, but if you apply it more often, your skin will feel a bit heavy and oily. I think this sunscreen is better for specific occasions rather than for daily use. If I had to choose a sunscreen for daily use, I would pick one with a lighter formula, like Fusion Water from Isdin.
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Reviewed in the United States on September 11, 2025
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