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Description
Human IFN-γ ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IFN antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IFN present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IFN-γ antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IFN-γ present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | Human interferon gamma ELISA kit, IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Gamma interferon (also known as type II interferon) is an important cytokine that can exercise immunomodulatory function. It was discovered for its antiviral activity. Interferon gamma plays a key role in host defense through antiviral, antiproliferative, and immunomodulatory functions. In many types of cells, gamma interferon induces cytokine production and upregulates the expression of different membrane proteins, including type I/II major histocompatibility complex (MHC-I, MHC-II), Fc receptors, leukocyte adhesion molecules, B7 family antigens. Gamma interferon can effectively activate macrophages and guide the synthesis, type transformation and secretion of B cell immunoglobulin. Gamma interferon can also affect the development of T helper cell subtypes by inhibiting the differentiation of Th2 and stimulating the growth of Th1. Gamma interferon plays an important role in some inflammatory disease processes such as autoimmunity and atherosclerosis. Biologically active gamma interferon is a non-covalently bound dimer with a molecular weight of about 20-25KD and varying degrees of glycosylation. Mature human interferon gamma has close to 90% homology with the amino acid sequence of interferon gamma from rhesus monkey, 59-64% homology with interferon gamma from cattle, dog, horse, cat and pig, and 37-43% homology with interferon gamma from cotton rat, mouse and rat. The interferon dimer first binds to the transmembrane receptor IFN-γRI (α subunit), and then binds to IFN-γRII (β subunit) to form a functional complex containing two α subunits and two β subunits; Wherein the IFN-γRII in the complex receptor can increase the affinity of the ligand as well as the signal transduction efficiency. Although the γ subunit is constitutively expressed in many types of cells, the β subunit cell expression regulates the influence of the response state of the receptor interferon γ and is strictly regulated. Interferon gamma can be secreted by many cells under a series of inflammatory conditions, including dendritic epidermal/γδ T cells, keratinocytes, peripheral blood γδ T cells, mast cells, neurons, CD8 + T cells, macrophages, B cells, neutrophils, natural killer cells, CD4 + T cells and testicular sperm cells, etc. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 9.38pg/mL-600pg/mL |
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4.6 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Dogs enjoy the treat on inside
Size: Medium (Pack of 3), Color: Orange, Green & Yellow, Pattern Name: Dog
My dogs love these. Better than chewing on rawhide
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 8, 2026
★★★★★ 5
Favorite bones for my dog
Size: Small, Color: Pink
Dog favorite bones, but broken fast pink bone. I buy multiple times.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 7, 2026
★★★★★ 5
Great for a teething puppy
Size: Small, Color: Pink
This is one of the best training collars we have used. So good that we ordered a second one so each dog has one and we have recommended it to several other people. It has 3 options, beeping, vibration and shock. Each option has several different settings. The collar comes in many different colors. It is very sturdy and it is waterproof. It is adjustable and the clip is very sturdy. I had previously bought a different training collar that did not phase my dog in the slightest and was a complete waste of money. It always seemed to be low on battery even after charging, this one however lasts like a week after charging. I will continue to use this brand for sure
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 8, 2026
★★★★★ 5
The Toy Annie Always Comes Back To
Size: X-Small, Color: Pink
⭐⭐⭐⭐⭐
These have become one of Annie's all-time favorite toys.
Annie is a tiny 4-pound dog, and she can be surprisingly picky about toys. She has a basket full of options, but she consistently comes back to her nubby Nylabones.
I think the size and texture are a big part of why she likes them so much. They're easy for her to carry around, chew on, and play with by herself. Whenever I buy a new one, it quickly becomes part of her regular toy rotation.
As a small-dog owner, I appreciate finding toys that are appropriately sized and actually get used instead of being ignored after a few days.
If you have a tiny dog who loves to chew, these are definitely worth trying. They've been a favorite in our house for a long time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 4
Dogs love these!
Size: Small, Color: Pink
My miniature Goldendoodle puppies love these! The only drawback is after several weeks of chewing, the bones develop pretty sharp edges long before the actual bone has been worn down. At that point, they are dangerous for the dog’s gums and must be thrown away.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 3, 2026
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