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Description
Mouse Gastric Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.6 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 4
Not Perfect but Nice 👋
Color: Shiny Black, Green Lenses, Size: Large
Looks like regular sunglasses. 🤙 The AI is pretty basic. The responses are basic. Battery life is fairly good and the case as a portable charger extremely cool. Why did I get these? FOR THE CAMERA.
The video recording is good. Good picture quality and this is the only camera glasses with image stabilization. When manufacturers make wearables recording video image stabilization is a must. Here its good. The footage is not shaky. But this coolness is only available in 1080p even though this records 2k or 2.7k. The app works fairly good but a tiny bit finicky as videos are not always loading to your gallery on phone or app automatically.
The app in general is cool in creating some videos but again basic and limited with restrictions.
The thing I don't like is connecting to an app which all these companies do to grab your data. Seems to be more about the app than the product.
They only things that bug me about this is the 5min recording limit and the flashing light that is a distraction to the person being filmed. There are no blinking lights on our cellphones when recording. You can turn off LED blinking lights on all cameras but here they claim some privacy issues.
When in public there is no right to privacy. When in private property and you suspect anyone recording with anything and you don't want it then say so other than than that there are cameras everywhere. They can record us but we can't record them? We don't need the blinking light and we need longer record times.
I would like a seperate SD slot so I don't have to connect to my phone all the time to pull footage. These aren't waterproof so... This is a quality product with good audio and video. Interested in any updates to turn off the blinking LED and recording time extensions.
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Reviewed in the United States on January 3, 2026
★★★★★ 5
Meta can handle it for you!
Color: Matte Black, Transitions Grey Lenses, Size: Standard
Absolutely AMAZING! Once you lern how its works, it's like having a personal AI that can help with almost everything you want in cyberspace. Questions, music, task, you name it, Meta can do it! Well worth the cost.
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Reviewed in the United States on June 6, 2026
★★★★★ 5
She loves them!!
Color: Matte Black, Clear Lenses, Size: Standard
Bought these for my daughter that is a content creator.. she does cooking and food reviews.. She absolutely loves these glasses!!!! They aloud her to free up her hands and not have to worry about holding her phone or using a tripod as much.. the video and picture quality is great.. I've heard her both listen to music and talk on the phone with them. They also look great on her. She is going to add her prescription lenses to them.. These meta gen 2 glasses are pricey ($322 plus tax on sale).. But if you creat content even at the regular price its a no brainer..
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Reviewed in the United States on June 8, 2026
★★★★★ 5
I use them daily
Color: Matte Black, Transitions Grey Lenses, Size: Standard
Enjoying arguing with the Meta AI, which is often wrong until you point it toward a current source of info.
Bought them for the headphones, which, like the Xreal AR Glasses. Have an open air implementation that allows hearing stuff around you but playing music others don't hear. And the sound while having little bass, is quite good.
The 3k hand free photo and video camera is a bonus, but only shoots in vertical, a stupid idea. But meant to push Instagram and Tic-Tic use.
In 3k you can blow things up to 16x9 and have ok results.
It also answers calls and will do as much as you connect it to.
You get the phone audio over the glasses for calls, playing YouTube videos or anything your phone plays, whether an app the AI uses or not.
I have Amazon Music and Audible attached so I can voice command them while driving, but Serious radio and YouTube music are fine, but you have to start them manually. The skip to next song glasses commands work on these apps too.
Overall a very entertaining device.
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Reviewed in the United States on April 23, 2026
★★★★★ 4
You’ll Experience at least one issue
Color: Matte Black, Clear Lenses, Size: Large
These are really nice but after about a month or two there are some issues. I ended up sending mine back cause connection issues. I recommend a protection plan.But when they dod work they were great.
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Reviewed in the United States on May 31, 2026