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Description
Mouse Gastric Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.5 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 1
Not what is expected
Size: Small, Color: Black-red, Size: Small, Color: Black-red
There are buttons on the back that go all the way down that personally in my opinion ruins the style!! Will be returning because that is not what I expected.
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Reviewed in the United States on January 20, 2026
★★★★★ 3
Faded Glory 😂 3.5 rating
Size: XX-Large, Color: Black-red, Size: XX-Large, Color: Black-red
Sooo faded I didn’t even want to try it on. And very dusty and ashy looking but only the red. It’s as if they had someone wear and wash it and return it and they took the red side and buttoned it onto another color and shipped it out. Guess these sellers like faded clothes. I wouldn’t buy certain colors seeing ppl talking about how the color doesn’t even last even upon its arrival. But if you have dye it’s your best bet to have some on hand when you order or even good for DIY projects.
Good thing is I did try it on and it fits and feels like good quality with good weight to it. May have to go up a size I’m a female with big breast. Seems like it may last, just not the color. Good stitch work buttons seem sturdy. Yea these shirts would be great for DIY work
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Reviewed in the United States on January 14, 2026
★★★★★ 5
Great jacket
Size: X-Large, Color: Black-red
My son loves this jacket
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Reviewed in the United States on January 19, 2026
★★★★★ 5
That it fits good and doesn't feel cheaply made
Size: 36W x 30L, Color: Blue
Very nicely made and comfortable to!
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Feel that flex without feeling that rubbery texture.
Size: 36W x 30L, Color: Black
These fit great! The texture isn't wuite like denim, more like a soft tablecloth, but that's quite alright. They have a flex that I need. Sometimes with "slim fit" stretch jeans, I feel a bit restrained in my thighs, this time, instead of ordering my usual size, I went up a notch. I can just wear a belt if I need to. I can fully articulate my legs in this set, in the stretchiness doesn't feel like weird rubbery velvet. They look good, they feel good. I imagine they'll last a bit longer than some of my other stretch jeans that had spandex or whatever in them.
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Reviewed in the United States on April 11, 2026