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Description
Human Liver Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, and cut the tissue into a tissue block with a volume of about 1-3mm3by using ophthalmic scissors or scalpel (4) the tissue is digested with human normal liver primary tissue digestive solution C, and then shaken for 10-20min at 37 ℃ (observe the digestion at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human normal liver organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids are collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human normal liver organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul human normal liver organoid culture medium a. |
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| Description | Human normal liver organoid culture medium is a medium that promotes the formation and expansion of human normal liver organoids in vitro. The product is a sterile liquid mixing system, containing amino acids, vitamins, organic and inorganic compounds and growth factors necessary to maintain the growth of target cells. The unique formula of this medium can provide a suitable nutritional environment for cells and promote the growth and expansion of human normal liver organoids in vitro Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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★★★★★ 4
Pretty shade; not super shiny
Color: Rose Gold
Pretty color! However- it will not be as pretty shiny as it looks like in the brand photos.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Works great
Color: Bronze Gold
Works great! Looks natural will buy more. Leaves a little sparkle
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Bello brillo corporal
Color: Silver, Color: Silver
Tu cuerpo queda con un brillo espectacular pareces modelo
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Reviewed in the United States on May 22, 2026
★★★★★ 5
shimmer
Color: Rose Gold
I recently tried the UCANBE Body Shimmer Oil, and I have to say, I'm really impressed! The oil has a beautiful, subtle shimmer that adds a touch of glow without being too overpowering. It feels luxurious on the skin, absorbing quickly and leaving it soft and smooth. I love how it gives my skin a healthy, radiant look, especially when I wear it on my legs and arms. The scent is light and refreshing, not too strong, which is a plus for me. Overall, it's perfect for a little extra sparkle on special occasions or just when I want to feel a bit extra on a regular day! Definitely recommend it if you're looking for a quality shimmer oil that doesn't feel greasy.
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Reviewed in the United States on April 1, 2025
★★★★★ 1
Bad Batch
Color: Gold
It was so thick & stinky. Had to toss it and take a shower right away. Couldn’t get it off my skin.
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Reviewed in the United States on February 28, 2026