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Description
FITC Mouse Anti-Human CD20 Antibody (S-R449)Product Specification Host Mouse Antigen CD20 Synonyms B lymphocyte antigen CD20; Bp35; Leukocyte surface antigen Leu 16; MS4A1 Location Cell membrane Accession P11836 Clone Number S R449 Antibody Type Mouse mAb Isotype IgG2b,k Application FCM Reactivity Hu Positive Sample human PBMC Purification Protein A Concentration 0. 2 mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin 300 Stability &
Product Specification
| Host | Mouse |
| Antigen | CD20 |
| Synonyms | B-lymphocyte antigen CD20; Bp35; Leukocyte surface antigen Leu-16; MS4A1 |
| Location | Cell membrane |
| Accession | P11836 |
| Clone Number | S-R449 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
B-lymphocyte antigen CD20 or CD20 is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. Immunohistochemistry can be used to determine the presence of CD20 on cells in histological tissue sections. Because CD20 remains present on the cells of most B-cell neoplasms, and is absent on otherwise similar appearing T-cell neoplasms, it can be very useful in diagnosing conditions such as B-cell lymphomas and leukaemias.
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Exchange/Return Notes
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