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Description
Mouse CNR1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Cannabinoid Receptor 1, Brain (CNR1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Cannabinoid Receptor 1, Brain (CNR1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Cannabinoid Receptor 1, Brain ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cannabinoid receptor type 1, also known as cannabinoid receptor 1, is a G protein-coupled cannabinoid receptor encoded by the CNR1 gene. Disorders associated with CNR1 include cannabis dependence and abuse. Pathways involved in this gene include peptide ligand binding to receptors and GPCR signaling. Gene Ontology (GO) annotations associated with this gene include G protein-coupled receptor activity and cannabinoid receptor activity. An important homologue of this gene is CNR2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.1 ★★★★★
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★★★★★ 5
Lo recomiendo
Color: BCMY
De lo mejor
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Reviewed in the United States on April 29, 2026
★★★★★ 5
Vibrant Creations Await
I recently ventured into the world of sublimation printing, and my experience has been nothing short of fantastic, thanks to the Hiipoo 500ML Sublimation Ink. This ink has proven to be a game-changer, elevating my creative projects with its exceptional quality and vibrant results.
One of the standout features of the Hiipoo Sublimation Ink is its impressive color reproduction. The ink delivers rich and vivid hues that truly pop, allowing me to bring my designs to life with unparalleled vibrancy. Whether I'm working on textiles, ceramics, or other substrates, the results are consistently stunning, making every print a true masterpiece.
The ease of use of this sublimation ink is commendable. The 500ML size provides ample ink for extended printing sessions, reducing the need for frequent replacements. The ink flows smoothly through my printer, ensuring precise and even distribution on the transfer paper. The compatibility with various sublimation-ready surfaces adds to the versatility of this ink, making it suitable for a wide range of creative projects.
Durability is a key factor that sets Hiipoo Sublimation Ink apart. The prints produced with this ink boast excellent longevity, resisting fading and wear over time. This ensures that my creations maintain their brilliance and sharpness, even after numerous washes or exposure to the elements.
I appreciate the meticulous attention to detail in the formulation of this sublimation ink. It dries quickly, preventing smudging and allowing for efficient production without compromising on quality. The consistent color output across different batches of ink speaks volumes about the reliability and quality control maintained by Hiipoo.
The cost-effectiveness of the Hiipoo 500ML Sublimation Ink is worth noting. The ink offers an excellent balance between quality and affordability, making it an ideal choice for both hobbyists and professionals alike. The value for money is evident in the impressive results it consistently delivers.
In conclusion, Hiipoo 500ML Sublimation Ink has truly exceeded my expectations, providing a reliable and high-quality solution for my sublimation printing needs. If you're looking for sublimation ink that delivers on both color vibrancy and durability, Hiipoo is the brand to trust. Elevate your creative projects with this exceptional ink and unlock a world of vibrant possibilities.
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Reviewed in the United States on February 5, 2024
★★★★★ 5
The best inks! And the best deal! Love it 🎨🖨️
Such a great deal for the price! You get all four colors + an extra black! It also comes with gloves and the syringes to use to fill the ink into your printer. ✨
And it was also nice that the company included heat tape, which is always needed for sublimation crafters! 💕 (I have not used the tape yet so I can’t speak on how well it works but when I do, I will definitely update my review to let you guys know how it went. 😊)
I love the Hiipoo brand inks—I’ve used them for years and it never fails. I will only use the Hiipoo ink. They are the best! 🎨🖨️
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Reviewed in the United States on February 16, 2026
★★★★★ 5
Great for Sublimation & DTF films
Great ink for my Epson 15000 printer for Sublimation. Also the ink is so vibrant printing DTF films for your DTF projects. I do many projects with Sublimation & DTF that I haven't refill with new ink yet. Beautiful colors that I love every single time I print.
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Reviewed in the United States on March 17, 2026
★★★★★ 5
Great Ink
Great ink that’s been bought by my whole family for sublimation.
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Reviewed in the United States on May 25, 2026