SKU: 96502835660

Poly(A) Polymerase Tailing Kit

Sale price$189.00 Regular price$210.00
Save 10%

Pay in installments of $52.50 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 27 - Aug 1

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Poly(A) Polymerase Tailing KitProduct Specification Stability & Storage Store at 25 ~ 15 for 2 years Reference Components UA070116 50 Rxns UA070116 250 Rxns E. coli Poly(A) Polymerase (5 U l) 100 l 5 100 l 10 X PAP Reaction Buffer 300 l 5 300 l 10 mM ATP 300 l 5 300 l RNase Free dH2O 1 ml 5 1 ml Protocol 1. Prepare the following reaction system on ice according to the table below: Component Volume Final Concentration 10 X PAP Reaction Buffer 5 l 1 X RNase Inhibitor (40U l) 1. 25 l

Product Specification


Stability & Storage

Store at -25 ~ -15℃ for 2 years

Reference


Components

组分

UA070116-50 Rxns

UA070116-250 Rxns

E. coli Poly(A) Polymerase

 (5 U/µl)

100 μl

 5 × 100 μl

10 X PAP Reaction Buffer

300 μl

5 × 300 μl

10 mM ATP

300 μl

5 × 300 μl

RNase Free dH2O

1 ml

5 × 1 ml


Protocol

1. Prepare the following reaction system on ice according to the table below:

Component

Volume

Final Concentration

10 X PAP Reaction Buffer

5 μl

1 X

RNase Inhibitor (40U/μl)

1.25 μl

1 U/μl (Optional)

E.coli Poly(A) Polymerase

(5 U/μl)

2 μl

0.2 U/μl

10 mM ATP

5 μl

1 mM

RNase Free dH2O

Up to 50 μl

-

Gently pipette to mix thoroughly. 2. Poly(A) tailing reaction: Incubate at 37°C for 30–60 min. 3. Reaction termination: Immediately store at –20°C after completion, or add EDTA to a final concentration of 10 mM, or perform phenol/chloroform extraction followed by ammonium salt/ethanol precipitation of RNA.

Guidelines

1. Due to the involvement of RNA manipulation, strict adherence to RNA operation protocols is required to avoid RNase contamination. Related reagents and consumables must be treated with DEPC to remove RNase or ensured to be RNase-free. 2. The RNA used for the tailing reaction should be appropriately purified before use and dissolved in RNase-free dH2O. The solution should not contain EDTA or salts. 3. If it is difficult to ensure a strictly RNase-free environment, it is recommended to add an appropriate amount of RNase Inhibitor to the reaction system to enhance the stability of RNA in the solution. 4. During experimental operations, enzyme products should be kept on ice at all times and stored at -20°C immediately after the experiment.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 96502835660

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.2 ★★★★★
Based on 19 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
L
Verified Purchase
Lyn
Whiting, US
★★★★★ 5
Super cute
The shelf is cute, and super easy to set-up. Be careful with the screws, I stripped the magnet screw/hole a little bit. Street that I was a little weary on over-tightening the four corners of each shelf. Make sure you use the wall straps bc it will be a little wobbly.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 17, 2026
D
Verified Purchase
DeeDee
Dallas, US
★★★★★ 4
Great shelving unit
Great shelving unit. Caution as there is no backboard to the shelves.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 15, 2026
M
Verified Purchase
Morgan
Dallas, US
★★★★★ 5
Great shelf
Color: Greige, Size: 6 Tier(10.7 x 15 x 65.3 Inches), Color: Greige, Size: 6 Tier(10.7 x 15 x 65.3 Inches)
Very impressed with this! It was very easy for me to put together without any help. Very sturdy pieces and seems well made. Really can’t beat the quality for the price! The glass door on the bottom really dresses up this shelf. I will probably buy more of these for other rooms in my house.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 2, 2025
S
Verified Purchase
softasylum
Dallas, US
★★★★★ 5
Elegant shelf, easy assembly!
Color: White, Size: 4 Tier(10.7 x 15 x 40.8 Inches), Color: White, Size: 4 Tier(10.7 x 15 x 40.8 Inches)
I almost returned this because I didn’t think I could put it together, but the step by step picture instructions were easy to follow, and I assembled this in about 50 minutes, taking my time and also wiping down each wood piece so it would be ready to go once finished. This came packed very well and no parts were missing or messed up. It’s very elegant looking for being inexpensive and just what I needed budget wise and style wise. It is sturdy and beautiful and I like it very much and am glad I kept it, it is worth it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 1, 2024
K
Verified Purchase
Kim N
Dallas, US
★★★★★ 5
Looks good, lightweight
Color: Brown, Size: 6 Tier(10.7 x 15 x 65.3 Inches), Color: Brown, Size: 6 Tier(10.7 x 15 x 65.3 Inches)
Very easy to put together. Lightweight, great for what I was looking for. Holds a lot of books and perfect for me. I didn't want a heavy clunky bookcase.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026

recommand products