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Description
MCP-2/CCL8 Protein, HumanProduct Specification Species Human Synonyms HC14,Small inducible cytokine A8 Accession P80075 Amino Acid Sequence Gln24 Pro99 Expression System E. coli Molecular Weight Approximately 7. 8 kDa, a single non glycosylated polypeptide chain containing 70 amino acids. Purity 97% by SDS PAGE or HPLC. Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized powder Storage Buffer 20 mM PB, with 150 mMNaCl, pH 7. 4. Reconstitution Before use this
Product Specification
| Species | Human |
| Synonyms | HC14,Small-inducible cytokine A8 |
| Accession | P80075 |
| Amino Acid Sequence | Gln24-Pro99 |
| Expression System | E.coli |
| Molecular Weight | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Purity | ＞97% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20 mM PB, with 150 mMNaCl, pH 7.4. |
| Reconstitution |
Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2) , is belonging to the CC chemokine family hat signal through CCR2 and, with the exception of MCP-1, other CCR receptors. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7) . These three MCPs were found by IL-1- beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells but not monocytes or neutrophils. The MCP family cross-reacts across species. Recombinant Human MCP-2 (CCL8) is an 8.9kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
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