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Description
Rat sAA1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 50 ng/mL). Then dilute to the following concentrations: 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, 1.5625 ng/mL, 0.78125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 50ng/mL standard working solution into the first EP tube and mix thoroughly to make a 25ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Amylase Alpha 1, Salivary (sAA1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Amylase Alpha 1, Salivary (sAA1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Amylase Alpha 1, Salivary ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Salivary amylase alpha 1 (sAA1) is an enzyme whose gene is found in many organisms. Amylase is a secreted protein that hydrolyzes 1,4-α-glucosidic bonds in oligosaccharides and polysaccharides, catalyzing the first step in the digestion of starch and glycogen in food. Several amylase genes are found in a cluster in the genome, expressed at high levels in the salivary glands or pancreas. It is an isoenzyme of amylase produced by the salivary glands. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.78-50 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.7 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
Helps with self tanner application
Color: back mitt
Received quickly and works as expected. Definitely helped me apply self tanner to my back and shoulders!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 6, 2026
★★★★★ 5
Glowing Skin
I’ve been using this PDRN Salmon Egg Serum for a short time, and I’m genuinely impressed. My skin looks noticeably plumper, calmer, and more radiant, almost like it’s been well-rested and deeply nourished from within.
The texture is lightweight and silky, absorbs beautifully, and layers perfectly under moisturizer without any stickiness. What stood out most to me was how soothing it felt—my skin looked less stressed and more balanced after just a few uses. Fine lines appeared softer, and my overall complexion had a healthy glow that didn’t rely on makeup.
I also love that it delivers visible results without irritation, which is rare for regenerative-style products. It feels like a professional treatment bottled into a daily serum—luxurious, effective, and worth every drop.
If you’re looking for a serum that supports skin repair, elasticity, and glow, this one is truly a gem. I’ll definitely be keeping this as a staple in my skincare routine. ✨
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Reviewed in the United States on December 26, 2025
★★★★★ 5
Glow-Up in a Bottle!
I’m thrilled with my experience using the FRANKLY Salmon PDRN Bounce Ball Serum. From the packaging to the results, everything has felt premium and thoughtful.
What I love:
The “bounce ball” concept is so fun — you shake it, the orange capsule breaks into micro-particles, and it disperses beautifully into a silky essence.
The texture is lightweight and non-sticky. It absorbs fast without leaving a greasy film, which makes it perfect under makeup or SPF.
My skin feels more plump and elastic after a week of regular use. I’ve noticed a subtle radiance and firmness that wasn’t there before.
The formula is fragrance-free, non-comedogenic, and avoids many common irritants, which is great since I have sensitive skin.
I appreciate that it layers well. It plays nicely with my other skincare steps without pilling or interfering.
A few tips / caveats:
After several uses, the serum may look a bit hazy or cloudy. That’s normal — repeated shaking can break up the ball more and cause the look, but performance isn’t affected.
Sometimes the bounce ball may temporarily separate or float after shipping or rough handling; just shake it again, let it rest, and it will reform.
Because it’s fairly concentrated, a little goes a long way. Use sparingly and let it fully absorb before applying thicker creams.
Bottom line:
This is one of those serums that feels like a small treat every time you use it. My skin feels better—more plump, hydrated, and glowing—and I’ve gotten compliments already. If you’re curious about a playful, high-performing K-beauty serum, this one is worth trying.
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Reviewed in the United States on October 9, 2025
★★★★★ 5
Love, Love, Love!
This serum is worth every penny. It leaves my mature, dry skin silky and moisturized without any stickiness. I’ve tried many oily serums, but this is the only one that dries to a soft, silky finish. I've noticed significant improvements in my skin texture. It’s an essential addition to everyone’s daily routine.
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Reviewed in the United States on May 10, 2026
★★★★★ 4
Bought it for the Egg Yolk Ball
Be real, the egg yolk ball caught your eye. If not, well, it did for me. I read the list of ingredients and they seemed pretty good so I figured I would give it a try. The directions were pretty simple: Shake the bottle like crazy and then apply. I only just started using it so I can't attest to how it works on fine lines, which is why I gave it four stars.
I will say that it hasn't made me break out and I LOVE that it seems to melt right into the skin. It doesn't stay like some sticky mask, which is a feeling I really loathe. I also really like that there's absolutely no scent to it. After using it about two times I do feel like my skin is a little softer and more hydrated. My skin also has this really nice "glassy" look to it, which I love. Normally, the only way I get that is if I have some really heavy hydrating cream that makes me feel super gross. I'd say the glassy look lasts a few hours.
Will come back and give this five stars after a month IF it that little ball thing really does work magic.
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Reviewed in the United States on July 29, 2025