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Description
Rat NDRG2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with protein NDRG2 (NDRG2) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of protein NDRG2 (NDRG2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Protein NDRG2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Nmyc downstream regulated gene 2, also known as NDRG2, is the protein encoded by the NDRG2 gene. This gene is a member of the N-myc downregulated gene family, belonging to the α/β hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in the development of glioblastoma and aggressive meningioma carcinomas (through cell proliferation). Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.0 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Revolutionary Health Guide That Actually Transforms Daily Wellness Through Practical Science!
Format: Hardcover
Glucose Revolution has been absolutely transformative for my health understanding and provides exactly the actionable, science-based approach needed to optimize energy, weight, and overall wellness through blood sugar management that actually works in real life! As someone who struggled with energy crashes, weight fluctuations, and general wellness concerns while wanting evidence-based solutions rather than fad diet approaches, this book delivers the comprehensive education and practical strategies that make sustainable health improvement achievable through understanding glucose impact on daily functioning.
The scientific foundation is outstanding with clear explanations of how blood sugar affects energy, mood, weight, and long-term health while making complex metabolic concepts accessible to readers without medical backgrounds. The research-based approach provides confidence that recommendations are grounded in genuine science rather than marketing trends or unsubstantiated claims.
What impressed me most is the practical application focus that translates scientific knowledge into daily habits and food choices that actually work within busy lifestyles and real-world eating situations. The actionable strategies eliminate the gap between health knowledge and implementation that makes many health books intellectually interesting but practically useless.
The meal timing and food combination guidance provides specific, implementable advice about optimizing glucose response through strategic eating patterns that don't require extreme dietary restrictions or complicated meal planning. The flexible approach accommodates various dietary preferences while delivering consistent metabolic benefits.
The energy stabilization benefits have been genuinely life-changing with elimination of afternoon crashes and dramatic improvement in sustained energy throughout the day that supports productivity and overall quality of life. The consistent energy demonstrates real metabolic improvement rather than temporary dietary effects.
The weight management insights explain the connection between glucose spikes and weight gain while providing sustainable approaches to weight control that don't rely on calorie restriction or unsustainable eating patterns. The metabolic approach to weight management proves more effective than traditional dieting methods.
The food selection strategies help identify which foods support stable blood sugar while showing how to modify meals and snacks to minimize glucose spikes without eliminating entire food groups or following restrictive eating plans that become impractical for long-term adherence.
The lifestyle integration makes glucose optimization practical within normal social eating, dining out, and family meal situations where extreme dietary approaches become socially isolating or practically impossible to maintain consistently over time.
The educational value extends beyond glucose management to comprehensive understanding of nutrition science and metabolic health that supports informed food choices and health decisions across various wellness goals and life circumstances that require adaptable health knowledge.
The motivation enhancement comes from visible, measurable results that demonstrate the effectiveness of glucose management approaches while providing sustainable motivation for continued healthy choices that don't rely on willpower or temporary enthusiasm that fades over time.
The long-term health impact addresses chronic disease prevention through metabolic optimization while providing proactive health strategies that support aging well and maintaining vitality throughout life rather than reactive approaches that address problems after they develop.
The accessibility makes advanced nutritional science understandable and applicable for readers regardless of previous health education while providing depth sufficient for those wanting comprehensive understanding of glucose metabolism and its health implications.
The value for transformative health improvement is exceptional for a book that provides education and strategies equivalent to expensive nutritional counseling while delivering lasting knowledge that supports lifelong wellness enhancement through practical, sustainable approaches.
For individuals seeking evidence-based health improvement that addresses energy, weight, and overall wellness through practical strategies that work within real-life circumstances while providing genuine understanding of metabolic health, this book delivers exactly the scientific foundation and actionable guidance needed for sustainable health transformation without extreme measures or temporary solutions.
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Reviewed in the United States on November 29, 2025
★★★★★ 5
Let me very clear. This book saved my Life!
Format: Hardcover
After a surprise diagnosis of Diabetes right before my retirement, this is the first book a bought after seeing segments on YouTube with the science of eating. I lost 60 pounds, got my glucose under control in less than 3 months and threw away all my diabetes medication! After three months, My doctor came in shaking my hand with congratulations! I was blown away. The book is full of practical advice for what to eat, when to eat, and what order to eat parts of your meal to control your blood glucose and not succumb to Diabetes. Life is all about choices! This book has easy instructions, thorough explanations, a little tongue in cheek humor along the way. If you want to kick your downward health spiral and kick diabetes to the curb making good health choices, then this is the Book for you.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2026
★★★★★ 4
Worth reading to better understand blood sugar, but don’t limit yourself to this one resource
Format: Hardcover
My mother had type 2 diabetes and ultimately died from related kidney failure. For years I have exercised and managed my diet in an effort to avoid a similar fate. I was therefore stunned during a recent doctor's appointment when it was revealed that my hemoglobin A1c was above normal limits. I immediately ordered a continous glucose monitor and was dismayed to see that my blood glucose levels were regularly exceeding what would be considered normal. I am now trying to learn as much as possible about preventing these elevated glucose levels.
In my research I was led to Jessie Inchauspe (Glucose Goddess on Instagram) and decided to buy her book, The Glucose Revolution. I will say that the majority of the information in this book is available for free on her Instagram account. However, I like the simplicity of reading through the book rather than scrolling through posts and reels.
The author gives a thorough overview of glucose, explaining the different forms and how they act in our bodies. She explains glucose spikes and has a really good explanation of what the hemoglobin A1c test is measuring. She then shares 10 hacks to help lower your blood sugar levels that I feel are relatively easy to incorporate into your eating plan. Some of them, like eating dessert over a sweet snack (eat a sweet food after a meal rather than on its own) were things that I have already been doing intuitively.
I do wonder at the description of our stomachs as a sink and the intestine as the pipe below. She claims that when we eat carbs first, they flow uninterrupted into the intestine. Drawing on information from my college nutrition classes many years ago, I seem to remember that chemical digestion begins in your mouth and that because of the churning in your stomach, the food that you eat will all be mixed around and therefore it doesn't really matter what order you eat your foods in. That being said, it's a relatively minor thing to eat a veggie starter and/or eat foods in the described order.
I also question the idea that our blood sugar shouldn't rise more than 30 points after eating. If my blood sugar is at 80, that means after eating my glucose level should not be above 110. That is well below the generally accepted rise to 140. I'm not saying that we should always be going to that level, but even a modest rise to 120 would not be accepable according to the standards presented in the book.
Of note, the author does not have diabetes or prediabetes and started the Glucose Goddess after working for a company that was developing continuous glucose monitors.
Overall, I do think this book is worth reading to better understand blood sugar. I wouldn't limit your knowledge to just this one source of information though. I've been slowly working through articles and videos published on the Nourished by Science website. I really appreciate the perspective offered there. Women entering menoupause might want to investigate the effects of decling estrogen on their blood glucose levels too. The New Menopause by Dr. Mary Claire Haver is a fantastic book on this stage of life.
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Reviewed in the United States on September 18, 2024
★★★★★ 5
Glucose Revolution is very healthy and has specific recommendations
Format: Hardcover
This was recommended by an outstanding organization that addresses discomfort and overall health and wellbeing, Pearson and Weary. This gives recommendations to stabilize glucose and flatten curves. There was beneficial discussion of the use of fiber which slows the body's glucose absorption. Comparisons of orange juice (lacks fiber) vs. eating an orange (includes fiber) that does not create a glucose spike. Evaluated sugar as a cause of not feeling well and leading to glucose spikes which can lead to heart disease, type II diabetes and cognitive decline. Glucose spikes also lead to inflammation which is a common source of illnesses: strokes, chronic respiratory disease, heart disease, liver disease, diabetes and obesity. When glucose curves are flattened the body burns fat. The book reviews the short term and long-term effects of glucose spikes. Cornell University which I attended as an undergraduate stated there is a specific order to reduce glucose spies by 73% that includes fiber 1st, protein and fat 2nd, starches and sugars last. Other recommendations: (1) never drink fruit juice as sugar from fruit is missing the fiber "resulting in disastrous blood glucose levels." (2) Breakfast cereals is one of the "worst ways to begin your day as it always creates tremendous glucose spikes." Choose savory breakfast can include yogurt, meat, cream cheese, protein powder, butter, eggs, and seeds. Toast alone increases glucose spikes but added peanut butter or avocado can minimize spike. (3) artificial sweeteners can increase insulin rise and havoc in the gastrointestinal system. (4) fasting has benefits for 8-12 hours. (5) snacking increases obesity. (6) add one tablespoon of apple cider vinegar to a large glass of water which is best 20 minutes before eating. This approach after 3 months led to a loss of 2-4 pounds. Vinegar stimulates mitochondria to burn more fat. (7) exercising after eating can also reduce glucose spike and is beneficial within 70 minutes of food consumption.
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Reviewed in the United States on December 9, 2025
★★★★★ 5
READ THIS AND CHANGE YOUR LIFE FOR THE BETTER NOW!!!
33% of Americans are verging on type 2 diabetes and do not know it.
This should make you run to get this book!
Following Glucose goddess on Instagram and got her book as well. It is easy to read, with fun illustrations by the author. She has truly helped me so much with my insulin resistance and managing my glucose spikes. "Lifestyle optimization" is an essential for everyone. Why wait until you are diagnosed? These hacks are simple, fun, and super easy. Her audiobook is also out now and is read by the author!! Which is awesome.
What makes this book even MORE useful though, is if you invest in a continuous glucose monitor (CGM) and have the app. I had the Freestyle Libre sensor. Each sensor lasts 2 weeks on the skin and logs your real-time glucose levels 24/7. Way better than doing the old fashioned painful and wasteful lancet and test strips. Although you can verify your CGM occasionally using the lancet, and see how accurate the CGM is. You can have a CGM prescription written for you if you INSIST at your nurse practitioner's. (NPs are WAY more open to this kind of experimentation and self-monitoring...I got the rebuff from the doctor, HA!)
What is so sad is that the healthcare system is so broken that insurance will NOT cover a CGM UNTIL you have full-blown type 2 diabetes and are dependent on exogenous insulin delivery. OR if you are a type 1 diabetic and born without the ability to process glucose. Sad huh? The FDA wants you sick, fat, ugly, and miserable. We are MUCH better desperate customers then...willing and eager to take any pills to solve the problems!
Let's not be proactive and encourage the PATIENT to take measures against type 2 diabetes, right?
So there are MANY benefits of managing glucose. Fairly effortless weight loss actually. In this book she dives into nutrition science and the biochemistry of different sugars as well. She has so much credibility and science-driven data backed up by her own personal journey as well as many peer-reviewed articles.
Thank you Jessie!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 20, 2022
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