Pay in installments of $63.89 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 28 - Sep 2
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat TNNI1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 2000pg/mL). Then dilute to the following concentrations: 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 2000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1000pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Troponin I Type 1, Slow Skeletal (TNNI1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the level of Troponin I Type 1, Slow Skeletal (TNNI1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Troponin I Type 1, Slow Skeletal ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Skeletal muscle slow-twitch troponin I (TNNI1) is a protein encoded by the TNNI1 gene. It is a tissue-specific isoform of troponin I, which is part of the troponin complex. TNNI functions to control the contraction and relaxation of skeletal muscle. Troponin I interacts with all major regulatory proteins in the sarcolemmal thin filaments of cardiac and skeletal muscle: troponin C, troponin T, myosin, and actin. When cell membrane Ca2+ levels are low, TNNI binds to the thin filaments, blocking the myosin binding sites on actin. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 31.25-2000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 18 reviews
Sort
Product Reviews
★★★★★ 1
These balls SUCK! External squeakers, minimal bounce and blue that is too dark.
Color: Multi Squeaker Balls, Size: X-Small, Style: 8-Pack
We have 2 little dogs in our home - a 1.5 year old Pomsky, and a 9 year old Pekingese. My Peke has shown no interest in tennis balls for years, until today! Imagine my excitement! Until she promptly destroyed the first squeaker on one of the blue balls. When I removed it, she began peeling the logos off the ball, and then started tearing the fuzz off! My dog has never destroyed any toy in her life, so this was shocking! Then the Pomsky decided that she wanted to play fetch with the new balls - she has Golden Retriever energy - and decided to destroy a squeaker in the midst of playing! She also wasn't nearly as interested in these tennis balls as her other tennis balls that she inherited from my Peke.
1. STUPID design, with the squeakers sticking out of the balls (the don't sit flush), so they are asking to get chewed or pulled out by any dog, even elderly 10 lb dogs! And a 9 lb young adult.
2. They are difficult to squeak compared to their Kong tennis balls, hence why the Pomsky wasn't interested for long.
3. They don't bounce as much as the Kong tennis balls, either, which made them less than exciting for the athletic and agile Pomsky.
Meh. We wanted to get the smallest tennis balls we could get (1.5 inch) because neither dog will play with the 2 inch or bigger ones, but we also wanted to get colors they could easily see! The Pomsky clearly prefers the yellow balls to any other color (red, orange, green, darker blue, watermelon design), so we wanted to get like a bright blue or turquoise couple of balls to appeal to them! Well, my senior Peke liked the blue ones the most, but also liked the red and orange. Pomsky didn't like the blue ones. I think they're too dark of a blue to be easily seen in our home afternoons and evenings in the winter.
We love Outward Hound toys in our house, but these were an expensive FLOP. They are the right size, but WHY did they put the squeakers so visible and appealing to dogs? I've never seen the squeaker in the Kong tennis balls.
My advice is that Kong seems to make the best tennis balls for toy breeds, in the 1.5" size. Outward Hound dropped the ball here... no pun intended. The blue is darker than the photos depict (may be better in natural sunlight outdoors), the squeakers are external, and they barely bounce at all. Our next step will be to get the pressurized bright blue tennis balls from another brand, in hopes that the bounce and visibility will outweigh the lack of squeakiness. If those don't work, I guess we'll stick to the classic neon yellow Kong tennis balls! At my local Target, they only have multicolor packs of them, and the dark blue, red and green get completely ignored.
*Dogs can only see shades of blues, yellows and black, grey and white. While some dogs may not seem to care about color, our Pomsky clearly does. :(
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 21, 2023
★★★★★ 5
My Dog Loves Them
Color: Multi Squeaker Balls, Size: Small, Style: 8-Pack
These cute little squeaky balls are the perfect size for my Shih Tzu. She loves to chew on them and make them squeak all the time, so they are durable. I would purchase them again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 3
Not super durable but good squeak while they work
Color: Multi Squeaker Balls, Size: Large, Style: 4-Pack
My dog loves these but they break very quickly. I've got an older set that was incredibly durable, but these all stopped squeaking/got holes after the first or second play session
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 5
Endless Fun and Excitement: Outward Hound Squeaker Ballz Fetch Dog Toy Review
Color: Multi Squeaker Balls, Size: Medium, Style: 4-Pack
f you're searching for the ultimate entertainment solution for your furry friend, look no further than the Outward Hound Squeaker Ballz Fetch Dog Toy. As a proud dog owner, I can attest to the sheer joy and excitement that these toys bring to our canine companions. Here's why the Outward Hound Squeaker Ballz Fetch Dog Toy deserves all the praise:
Irresistible Squeaky Fun: The Outward Hound Squeaker Ballz are an absolute delight for dogs of all shapes and sizes. Each ball features an attention-grabbing squeaker that ignites your pup's natural instincts for play and exploration. The squeaky sound adds an extra layer of excitement to fetch games, encouraging hours of active and engaging playtime.
Durable Construction: Made from high-quality, durable materials, the Outward Hound Squeaker Ballz are built to withstand the rigors of enthusiastic play. Whether your dog loves to chase, fetch, or chew, these toys hold up remarkably well against rough handling and gnawing, ensuring long-lasting enjoyment and entertainment for your furry friend.
Versatile Play Options: With a convenient pack of four medium-sized balls, the Outward Hound Squeaker Ballz offer endless opportunities for interactive play and bonding with your dog. Whether you're playing indoors or outdoors, these versatile toys provide a fun and engaging outlet for your dog's energy, helping to promote physical activity and mental stimulation.
Bright and Colorful Design: The vibrant colors and eye-catching design of the Outward Hound Squeaker Ballz make them easy to spot and retrieve during outdoor play sessions. Their bright hues add an element of excitement to fetch games, keeping your dog entertained and focused on the task at hand.
Safe and Non-Toxic: As responsible pet owners, we prioritize the safety and well-being of our furry companions. The Outward Hound Squeaker Ballz are crafted from pet-safe materials that are free from harmful chemicals and toxins, providing peace of mind knowing that your dog is playing with a safe and non-toxic toy.
Affordable and Value-Packed: With four balls included in each pack, the Outward Hound Squeaker Ballz offer exceptional value for the price. You can keep multiple balls on hand for backup or share them with fellow dog owners, spreading the joy and excitement of playtime to canine friends everywhere.
In conclusion, the Outward Hound Squeaker Ballz Fetch Dog Toy is a must-have addition to any dog owner's collection of pet supplies. With their irresistible squeaky fun, durable construction, versatile play options, bright and colorful design, safety features, and unbeatable value, these toys bring endless joy and entertainment to dogs of all ages and breeds. Treat your furry friend to the ultimate playtime experience with Outward Hound Squeaker Ballz Fetch Dog Toy - you won't be disappointed!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 27, 2024
★★★★★ 5
Squeaky fun!!
Color: Multi-colored, Size: Small - 12 Pack
These are perfect they fit in my hyper pet shooter and they squeak too! My dogs absolutely love themZ. Seems to be good value and I think they will soften up over time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 28, 2026