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Description
Gentamicin OneStep Competitive ELISA KitProduct Specification Antigen Gentamicin Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Others Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 5. 6% Inter assay: 12. 0% Sample type Cell culture supernatant, Serum, Plasma Assay type Competitive Sensitivity 0. 012 ng ml Range 0. 05 ng mL 5 ng mL Recovery Cell culture supernatant: 103% Serum: 91% Plasma: 111% Assay time 60 minutes
Product Specification
| Antigen | Gentamicin |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 5.6%； Inter-assay: 12.0% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Competitive |
| Sensitivity | 0.012 ng/ml |
| Range | 0.05 ng/mL – 5 ng/mL |
| Recovery | Cell culture supernatant: 103% Serum: 91% Plasma:111% |
| Assay time | 60 minutes |
Background
Gentamicin is an aminoglycoside antibiotic. It became a critical therapeutic agent due to its broad-spectrum activity against Gram-negative bacteria (e.g., Pseudomonas aeruginosa, Escherichia coli, Klebsiella) and some Gram-positive pathogens. As a bactericidal drug, gentamicin inhibits protein synthesis by binding to the 30S ribosomal subunit, disrupting mRNA translation and causing premature termination or misreading of genetic code. Clinically, it is used to treat severe infections such as sepsis, pneumonia, intra-abdominal infections, and complicated urinary tract infections. It is often combined with β-lactam antibiotics (e.g., penicillins or cephalosporins) for synergistic effects. However, its use is limited by dose-dependent nephrotoxicity and ototoxicity (hearing loss, vestibular damage), necessitating careful monitoring of renal function and serum drug levels.
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