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Description
Rat IL-2 OneStep ELISA KitProduct Specification Antigen IL 2 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Rt Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied. Kit Precision Intra assay: 2. 7% Inter assay: 3. 8% Sample type Cell culture supernatant Assay type Sandwich (quantitative) Sensitivity 9. 254 pg ml Range 62. 5 pg mL 4000 pg mL Recovery Cell culture supernatant: 113% Assay time 60
Product Specification
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 2.7%； Inter-assay:3.8% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 9.254 pg/ml |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Recovery | Cell culture supernatant: 113% |
| Assay time | 60 minutes |
| Species reactivity | Rat |
Background
Rat IL-2 (Interleukin-2) is a 15–17 kDa glycoprotein and key T-cell growth factor primarily secreted by activated CD4+ T cells, NK cells, and dendritic cells. It drives T-cell proliferation, Th1 differentiation, and NK cell activity while also supporting regulatory T cell (Treg) function for immune tolerance. Signaling through the IL-2 receptor (IL-2Rαβγ) activates JAK-STAT pathways, promoting cell survival and expansion. Rat IL-2 shares ~70% homology with mouse IL-2 but differs significantly from human IL-2, requiring species-specific detection methods (e.g., ELISA, flow cytometry). It is crucial in research on autoimmunity (e.g., EAE), transplantation, and cancer immunotherapy, with dysregulation linked to immune disorders. Recombinant IL-2 has therapeutic potential but demands rat-specific reagents for accurate study.
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