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Description
Calponin-1 Recombinant Rabbit mAb (S-R161)Product Specification Host Rabbit Antigen Calponin 1 Synonyms Basic calponin, Calponin H1 smooth muscle, CNN1 Location Cytoskeleton Accession P51911 Clone Number S R161 Antibody Type Recombinant mAb Isotype IgG Application IHC P, IF Reactivity Hu, Ms, Rt Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300 Stability & Storage 12 months from
Product Specification
| Host | Rabbit |
| Antigen | Calponin-1 |
| Synonyms | Basic calponin, Calponin H1 smooth muscle, CNN1 |
| Location | Cytoskeleton |
| Accession | P51911 |
| Clone Number | S-R161 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:1000 | null |
| IF | 1:1000 | null |
Background
Calponin 1 is a basic smooth muscle protein that in humans is encoded by the CNN1 gene. The expression of CNN1 is specific to differentiated mature smooth muscle cells, suggesting a role in contractile functions. Calponin 1 is up-regulated in smooth muscle tissues during postnatal development with a higher content in phasic smooth muscle of the digestive tract. Calponin binds actin to promote and sustain polymerization. The binding of calponin to F-actin inhibits the MgATPase activity of smooth muscle myosin. Calponin binds F-actin through two sites at residues 144-162 and 171–188 in chicken calponin 1. The two actin-binding sites are conserved in the three calponin isoforms.
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