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Description
Alexa Fluor® 647 Mouse Anti-Human CD325 Antibody (S-2882)Product Specification Host Mouse Antigen CD325 Synonyms Cadherin 2; CDw325; Neural cadherin (N cadherin); CDHN; NCAD; CDH2 Location Cell membrane Accession P19022 Clone Number S 2882 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Positive Sample A172 Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months
Product Specification
| Host | Mouse |
| Antigen | CD325 |
| Synonyms | Cadherin-2; CDw325; Neural cadherin (N-cadherin); CDHN; NCAD; CDH2 |
| Location | Cell membrane |
| Accession | P19022 |
| Clone Number | S-2882 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | A172 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD325, also known as N-cadherin or cadherin-2, is a 130–140 kDa type I transmembrane glycoprotein belonging to the cadherin superfamily. It is a calcium-dependent cell-cell adhesion molecule that plays a crucial role in maintaining tissue architecture and regulating morphogenetic movements during development. The protein consists of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. The extracellular domains mediate homophilic interactions between adjacent cells, while the cytoplasmic tail interacts with catenin proteins, which link the cadherin complex to the actin cytoskeleton. CD325 is expressed in various tissues, including neurons, cardiac muscle, and some cancer cells, and is involved in processes such as neural development, cardiac function, and cancer metastasis.
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