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Description
Rat gSAP ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Gamma-Secretase Activating Protein (gSAP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Gamma-Secretase Activating Protein (gSAP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Gamma-Secretase Activating Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | γ-Secretase activating protein (gSAP), also known as protein pigeon homolog (PION), is a protein encoded by the gSAP gene. It regulates γ-secretase activity, specifically activating amyloid protein (APP) interaction to increase β-amyloid production without affecting the cleavage of other γ-secretase targets. The γ-secretase complex is an endoprotease that catalyzes the intracellular cleavage of integral membrane proteins. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.6 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
The Massive Valet That Elevated My Workspace
First things first: this drawer is massive! But in a good way. The UPERGO Desk Drawer Organizer Tray can be used either as an under-desk mounted drawer or a desktop valet-style tray. I use it for the latter. When I come into the office, I tend to drop everything into the valet tray, including my cell phones, wallet, keys, earbuds or IEMs, and other everyday carry items. I utilize multiple desks in my office, including a couple of L-shaped desks and a tech bench for hardware repair and product reviews. This desktop valet-style tray checks all the boxes for my needs.
Simply put, it transforms the workspace. It has multiple compartments for various items of different sizes. My cell phones fit in one space, my pens and pocket journal in another, and my watch in yet another. It's ideal for your EDC (Everyday Carry) items, for sure, and having that nearby helps declutter and save space on your desk. I aim to keep the top of my desk as clean as possible and utilize an under-desk tray for my keyboard. This setup is perfect when I need to write in my pocket journal on top of my desk or take top-down shots for any product review. Ideally, it's probably better as a tray under a large monitor stand, on a decent-sized nightstand, or mounted under a desk in a 3D-printed tray—but it performs exceptionally well in my use case as an accessible yet organized tray for my essential items.
The black steel/aluminum and walnut finish looks extremely good in any layout, even better if you already have other matching items from UPERGO's lineup, including their other desk organizers, pen holder, laptop holder, or headphone stand. If you don't already own any of their products or haven't seen them, I highly recommend checking them out. They are a great alternative to pricier options, and honestly, their quality is just as nice. I have multiple items from their lineup, and they all pair well together, keeping the aesthetic uniform, tidy, and elegant.
It has elevated what was previously a gaming desk to a professional productivity desk. The pictures don't do it justice, as I was utilizing this tech bench/product review desk for this purpose. However, they should give you a clear look at the size of this valet-style tray. This is precisely the point I want to emphasize: this tray is huge, and you must have the desk space for it if you're going to use it on top of the desk. Otherwise, it would be great for a nightstand or as a key holder for an entryway stand.
It is premium for its price, sturdy, and won't slide around much at all. I love its look and overall minimalist design. It keeps my items organized and declutters the area where I am using it. Considering its price point and materials, this is one of the best items if you're looking for a decluttering solution that also looks fancy and professional.
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Reviewed in the United States on July 13, 2025
★★★★★ 5
Sturdy, heavy duty, great appearance.
Perfect desk drawer organizer for professional desk. I have 4 of these to help organize my drawers. Wood and metal is very classy. Feels heavy and durable. Perfect size for any drawer. I double them up.
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Reviewed in the United States on April 10, 2026
★★★★★ 5
Perfect midcentury organizer
After spending literally hours searching for a catchall/organizer, I finally decided to get this one for my husband. I'm so glad I did. His dresser is much more organized and clean, fits vitamin box, colognes, glasses, keys, and complements the midcentury look of our home. I ended up buying one for myself to store my perfumes and water bottles.
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Reviewed in the United States on December 15, 2025
★★★★★ 5
Great Desk Organizer Trays!
I don't often post reviews on Amazon but these desk organizer trays really deserve a review. These are LITERALLY knock off of Balolo trays at a fraction of the cost with the same quality and craftsmanship as Balolo. I got them with the hope of been able to take the divisions out and glad that I did. Taken the divisions out to use them as paper trays is possible and it is super easy to do with a screw driver taking 4 screws from under the tray. Then you can use them as paper trays. I got 5, three I'm using them as paper trays and 2 as desk organizers. The small tray and the pen holder are also worth getting to complete your desktop/office setup.
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Reviewed in the United States on July 27, 2025
★★★★★ 5
Good quality tray - didn't fit my desired use case but I'll still keep it
I bought this tray in place of one that costs more than double the price so the value is there. However, this tray does not fit within the upper tray of my desk shelf - it's probably about 1mm too thick to fit. Despite this, I've decided to keep the shelf because the quality indeed is good, and I just placed the tray under the other side of the desk shelf sitting on the desk instead. It keeps a number of my frequently used desk items organized.
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Reviewed in the United States on November 23, 2025
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