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Description
Mouse Anti-Mouse CD45.2 Antibody (S-3398)Product Specification Host Mouse Antigen CD45. 2 Synonyms Ly 5. 2; LCA Location Cell membrane Accession P06800 Clone Number S 3398 Antibody Type Mouse mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse splenocytes Purification Protein A Concentration 2 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS pH7. 4 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied
Product Specification
| Host | Mouse |
| Antigen | CD45.2 |
| Synonyms | Ly-5.2; LCA |
| Location | Cell membrane |
| Accession | P06800 |
| Clone Number | S-3398 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
The CD45.2 protein is a receptor-type protein tyrosine phosphatase predominantly expressed on the surface of all nucleated hematopoietic cells in mice. It serves as a key genetic marker for distinguishing hematopoietic cell subsets in mice. As a common isoform of the CD45 antigen, CD45.2 participates in the transduction of T-cell and B-cell receptor signals by regulating the activity of Src family kinases. It plays a critical role in lymphocyte development, activation, and the establishment of immune tolerance. The allelic variation of its encoding gene, Ptprc, determines the difference between CD45.1 and CD45.2. This distinction is widely utilized in experimental research, such as hematopoietic stem cell transplantation and immune cell lineage tracing, making it a classic molecular marker for the identification of mouse models in immunological studies.
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