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Description
RFX5 Recombinant Rabbit mAb (S-3628-50)Product Specification Host Rabbit Antigen RFX5 Synonyms DNA binding protein RFX5; Regulatory factor X 5 Immunogen Synthetic Peptide Location Nucleus Accession P48382 Clone Number S 3628 50 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P Reactivity Hu Positive Sample HeLa, Jurkat, K562, Raji, HCT 116 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0.
Product Specification
| Host | Rabbit |
| Antigen | RFX5 |
| Synonyms | DNA-binding protein RFX5; Regulatory factor X 5 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P48382 |
| Clone Number | S-3628-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, Jurkat, K562, Raji, HCT 116 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
The RFX5 protein is a core subunit of a key transcription factor complex regulating adaptive immunity; its canonical function involves assembling with RFXAP and RFXANK into a heterotetramer (an RFX5 dimer bound to one RFXAP and one RFXANK molecule), recognizing the X-box motifs in MHC-I and MHC-II promoters via its N-terminal conserved domain, and utilizing its C-terminal domain to collaborate with NF-Y and other factors in stabilizing transcriptional enhanceosomes—deficiencies in this function lead to primary immunodeficiencies such as bare lymphocyte syndrome (BLS). The latest research revealed at the structural level that RFX5 also acts as a unique pioneer transcription factor: its extended DNA-binding domain (eDBD) directly forms hydrogen bonds with histones via the Glu102 residue, specifically targeting the SHL+2 position on nucleosomes. This induces localized DNA distortion and the dissociation of distal DNA from the nucleosome surface, thereby actively opening chromatin to enhance DNA accessibility and paving the way for subsequent transcription factor binding.
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4.3 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 4
What a delight to read!
Format: Kindle
I'm not quite sure what I expected when I plunked down my money and downloaded A Quiet Life in the Country. A modern variation of the Golden Age country house mystery? If that was it, I didn't get it. What I did wind up getting was a whole lot more. I will warn you ahead of time: if you are a fan of those classic mysteries, T.E. Kinsey's book may not be your cup of tea, and now I'll set about telling you why.
Kinsey has written a good, puzzling, multi-layered mystery that gives you a real feel for village life out in the English countryside at the turn of the last century. While the plot and the setting are first-rate, it's Kinsey's characters that make this book shine-- and what makes the book turn away from those classic mysteries.
Inspector Sunderland moves from being a stereotype to being someone rather special, which I always love to see (being sick to death of incompetent policemen in the books I read).
Lady Emily Hardcastle's background is very mysterious indeed, and I hope it takes several books to have all the details teased out for us to see. However, it is the relationship between Lady Emily and Florence that is the pièce de résistance of A Quiet Life in the Country. Their dialogue is so full of mutual admiration, wit, and humor that it is, quite simply, a delight to read. As Sunderland witnesses one such conversation, he asks, "Are you two a music hall act?" and you have to admit that he has a point. (If they were a music hall act, I'd pay to see them.)
If you're in the mood for something that's light and fun, something that teases your brain, takes you to the English countryside of a century ago, and makes you laugh, have I got a recommendation for you: T.E. Kinsey's A Quiet Life in the Country. I'm on to the second book!
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Reviewed in the United States on March 6, 2017
★★★★★ 3
are looking to settle in to the nice, quiet
Format: Paperback
Welcome to Littleton Cotterell,a small village in Glouchestershire. The year is 1908, and Lady Emily Hardcastle and her intrepid maid, Florence Armstrong, are looking to settle in to the nice, quiet, retired life after several years spent adventuring around the world.
Naturally, such plans are always disrupted. In this case, the two women discover a dead body hanging from a tree while out on a morning walk. The death is meant to appear as a suicide, but certain inconsistencies seen to point more towards murder. With the local constabulary over their heads, Lady Hardcastle and Flo must draw on their previous experiences to solve the murder before anyone else gets hurt.
The mystery goes off in fairly expected fashion, with the eccentric and kindly Lady Hardcastle relying on her witty and resourceful maid. There are suspicious characters and red herrings aplenty. And, naturally, the initial mystery gets wrapped up in several others in the course of the book.
I will say this for A Quiet Life in the Country: it does not take itself too seriously. The usual tropes of the cozy mystery are addressed with a wink and a nudge (such as one character explaining to Lady Hardcastle that the tiny, 30 person village she just moved to is actually the murder capital of the country). I appreciate the effort made to acknowledge all the commonly used bits that go into a cozy murder mystery, and it certainly helped to dispel a feeling of deja vu.
In all, if you’re looking for a nice, quick mystery with not one but two strong female leads, you could do far worse than to join Flo and Lady Hardcastle on their adventures.
A free copy of this book was provided via Goodreads Givaways in exchange for an honest review. A Quiet Life in the Country is currently available for purchase (and, at the time of this writing, free via Kindle Unlimited).
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Reviewed in the United States on November 23, 2016
★★★★★ 5
Pay no attention to Amazon's forced "one word" reviews. Read this review instead!
Format: Kindle
When led to this page for my review I found a series of leading questions which I found quite offensive. Don't put words in my mouth Amazon. I'm quite capable of giving my own ideas and views! For instance instead of the above four one word clap trap, I give you my review: "Full of surprises" indeed!
That being said, I absolutely adored "A Quiet Life In The Country". I take full advantage to read for free lots of book through Prime, but most of it is schlock. This is the gold piece found buried deep, and worth the search. It's fun and frothy reading at the level of P. G. Wodehouse and Georgette Heyer. I echo a hearty Amen! to those who found Flo and Lady Hardcastle the most diverting sleuthing duo to come along in ages. I want much much more! Well researched too, as I learned who in the upper crust sails (as in Ladyship) and an assortment of early 20th century sports figures like W. G. Grace and Jack Hobbs, and after googling pictures of same, got quite a giggle out of Grace being portrayed as Oscar Wilde.
This is actually my second read of this series, and it is even better than I remembered. In the second one you get even more behind the scenes with Lady H and Flo and it is a wild ride. These are first class offerings and I'm actually buying them all and can't wait until the newest comes out next June (2017)!
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Reviewed in the United States on January 3, 2017
★★★★★ 4
Well written. Funny...out loud gafawing funny. Fun. Well crafted plot. Wonderful characters.
Format: Kindle
What a lovely surprise!
A well written, well plotted. Funny. Fun. Mystery with two fresh and fine friends...Lady Hardcastle and Flo Armstrong , her erstwhile Lady's maid, Servant, Strong Right Hand Woman and friend...and...Inspector Sunderland, who finds the two so innovative and essential to the investigation of Two Murders and Some serious thefts that he is determined, and says so, that whenever they can he desires them to assist in future!
I so enjoyed this first book in the series I invested in the next four...so far!...in the series.
What fun...the women and now the Detective Inspector, as well, have such comical and engaging exchanges one finds herself (me) laughing so heartily she needs to pause and wipe her eyes repeatedly as she reads the story!
Would highly recommend this series to anyone who loves a good, well plotted mystery and a giddy, heartfelt laugh...well, series of laughs, at the same time.
Dialogue is often funny...though murder and mayhem are not!...and this is a careful blend of both....just sayin'.
What a wonderful edition to a huge genre of cozy and some not so cozy mystery books.
I recommend this series.
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Reviewed in the United States on June 7, 2024
★★★★★ 5
a quiet life???
This is a short story collection like Sir Arthur Conan Doyle and Agatha Christie have for Holmes and Poirot/Marple.
The characters remind me of the Phryne Fisher books. There are four stories in Book 1
A Quiet Life in the Country
Lady Emily Hartcastle and her maid Miss Florence Armstrong are enjoying some time in the country in the small
town of Littleton Cotterell when they come across a dead body. They find out that it is Frank Pickering, a local man
and it is thought that he committed suicide. But investigating, it seems that he has been murdered. Who
did it and why?
The Circus comes to town
Lady Hartcastle meets an old friend George who is the manager who for a circus that has come to
town. The next day, the juggler Hubert 'Huey' Parving is found dead mawled in a cage and then
others began to die. Who is behind this?
The Case of the Missing Case
Lady Hartcastle and Flo go to the engagement party of young Clarissa Farley-Strouds. The
next day, Nelson Holloway, the trumpet player with that night's entertainment - Roland Richman's
Ragtime Revue. Who killed him? As they investigate, the clues lead them to possible cursed
stolen jewel.
The Half-Death of Gunther Ehrlichmann
Florence recounts her life before and after meeting Lady and Lord Hartcastle as she and
Lady Hartcastle along with Lady Hartcastle's brother hunt down a killer.
Each story is tied into the other, but exciting! Must read!
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Reviewed in the United States on May 20, 2016