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Description
Mouse ANXA9 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Annexin A9 (ANXA9) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Annexin A9 (ANXA9) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Annexin A9 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Annexin A9 (ANX/A9) is a protein encoded by the ANXA9 gene. Annexins belong to a family of calcium-dependent phospholipid-binding proteins. Members of the annexin family contain four internal repeating domains, each of which includes a type II calcium-binding site. This calcium-binding site is required for annexin aggregation and cooperative binding to anionic phospholipids and extracellular matrix proteins. This gene encodes a diverged member of the annexin family in which all four homologous type II calcium-binding sites within the conserved tetrad core contain amino acid substitutions, resulting in loss of function. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.2 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 5
5 Star
Format: Hardcover
Rachel is a very fine writer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2026
★★★★★ 5
Informative
Format: Hardcover
Good read
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Reviewed in the United States on March 28, 2026
★★★★★ 5
If we care about racism and white privilege, what should we do?
Format: Kindle
One hundred and fifty-two years ago, slavery ended in the United States. And yet the tentacles of that time touch lives every day, all these years later.
What can be done to make things better? Michael Eric Dyson, a sociology professor at Georgetown University, and an ordained Baptist minister, suggests that white people who care about the lives of black people should make individual reparations. In his book, Tears We Cannot Stop …A Sermon to White America, Dyson says, “{Black people} built a legacy of excellence and struggle and pride amidst one of the most vicious assaults on humanity in recorded history. That assault may have started with slavery, but it didn’t end there. The legacy of that assault, its lingering and lethal effect, continues to this day. It flares in broken homes and blighted communities, in low wages and social chaos, in self-destruction and self-hate too. But so much of what ails us—black people. That is—is tied up with what ails you—white folk, that is. We are tied together in what Martin Luther King Jr. called a single garment of destiny. Yet sewed into that garment are pockets of misery and suffering that seem to be filled with a disproportionate number of black people.”
The book, unlike Dyson’s other scholarly works, takes the form of a worship service, and uses the concept of an extended sermon, or jeremiad, to lead the reader through confession, repentence, and redemption “through the long night of despair to the bright day of hope.” In Dysons’s view, “whiteness is a problem to be struggled with,” and his book is of inestimable value in grappling with the struggle.
The book speaks at length of police brutality against black people, and fervently tries to create empathy in white readers. It includes an extraordinary bibliography of books which give insight and voice to black history, oppression, pain, achievement, and lives.
And it speaks of reparations, and our responsibility as white beneficiaries of an unequal system, to take concrete actions to right the wrong, the change our country and the lives of our black sisters and brothers and their children.
Dyson is imaginative, and has many suggestions for how an individual or group “I.R.A.”—an Individual Reparations Account. We could buy books for black college students, overpay our black accountant or hairdresser, pay the black person who cuts our grass double the amount on the bill, give to the United Negro College Fund, and more. He suggests that faith groups consider giving 10% of their revenues to a church I.R.A. In an interview in the New York Times Magazine, Dyson says, “If the sermon ain’t making you a little bit uncomfortable, it ain’t effective. Look, if it doesn’t cost you anything, you’re not really engaging in change: you’re engaging in convenience. I’m asking you to do stuff you wouldn’t ordinarily do. I’m asking you to think more seriously and strategically about why you possess and what you possess…..you ain’t got to ask the government, you don’t have to ask your local politician—this is what you, an individual, conscientious, ‘woke’ citizen can do.
I have read many—though surely not all—of the books Dyson recommends. I have grappled with white privilege as a mother of black children, a fighter against apartheid, a civil rights activist, a human being. I have never read anything which more cogently offers “woke whites” a path to being a part of the change. I urge you to read Tears We Cannot Stop …A Sermon to White America, and to take your place in the pantheon of people who help this country grow beyond its racist past.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 23, 2017
★★★★★ 5
Powerful and especially so read aloud by the author.
Format: Audio CD
Hearing this powerful book read by Dr Tyson made it doubly understandable and enlightening. I needed to hear the experience, hurt and anger expressed so incredibly honestly and with the power of his emotion. I saw the CDs here at a reasonable price and I am so much more aware hearing rather than reading this book. Dr Tyson teaches powerful historic and current systemic racial experience such as every white person will do well to encounter. If you want to learn more than you already know, listen to these CDs on Black experience from Dr Tyson. Could be disturbing to anyone who does not want to learn the truth he teaches. It’s so packed with truth I will listen to it more than once more. Thank you, Dr Tyson.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 3, 2025
★★★★★ 5
brilliant, painful
Format: Hardcover
Timely, provocative, brilliant, painful, and, ultimately, a very necessary step toward authentic national healing and transformation. As a white, long-time fellow traveler, I am so grateful that Dr. Dyson has answered his calling to lead us toward redemptive justice with truth, decency and love--albeit, at times, necessary tough love. I am in my third read already and learning more and more each time.
Dyson's sermon serves as an exceptional read for believer and non-believer alike as it offers a framework and lexicon for us to engage with one another as we work our way through ongoing racially turbulent and polarized times. I am not a critic by trade so I will leave the professional analysis to the experts. I am a K-12 educator and seeker of social justice. I found this work both accessible and challenging in the best of ways. In my daily work, the evidence of the invention of whiteness, white innocence and white fragility and their pernicious impacts are ubiquitously abundant such that Dyson’s claims simply cannot be denied.
Dyson’s sermon is not always a comfortable read. That’s the point. It is a workout for the soul and psyche that results in the growing pains necessary for personal and collective liberation.
Thank you, Rev. Dr. Michael Eric Dyson, for opening your head and heart so broadly and deeply there is room enough for all of us in this racial healing process. The writing of this book is the ultimate act of truth and forgiveness. You have entrusted us with your most profound insights, personal experiences, and extend a vulnerability that humbles and arrests me. As you shared in a recent interview, "your trust in us grows out of forgiveness and the demand for truth for which is stands on, and the love it seeks to extend". Amen! I am in!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 30, 2017
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