SKU: 72171513135

UniOne® TR-FRET Human BCL6/CRBN PROTAC Binding Kit

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Description

UniOne® TR-FRET Human BCL6/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage Store the kit at 80C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze thaw cycle; labeled antibody solutions can undergo oneadditional freeze thaw cycle. To avoid repeated freeze thaw cycles, it isrecommended to aliquot the remaining stock solutions into single use

Product Specification


Host Human
Stability & Storage

Store the kit at -80°C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze-thaw cycle; labeled antibody solutions can undergo oneadditional freeze-thaw cycle. To avoid repeated freeze-thaw cycles, it isrecommended to aliquot the remaining stock solutions into single-use containersfor storage at -80°C. Thawed diluents and Detection Buffer can be stored at2-8°C.

Background

This kit uses Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the molecular glue ARV-393 and testcompound-mediated interaction between the DDB1/CRBN complex and BCL6. Thismethod enables simple and rapid high-throughput screening of small moleculescapable of mediating the interaction between the DDB1/CRBN complex and BCL6.

As shown, the interaction between DDB1/CRBN and BCL6 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the molecular glueARV-393 mediates the interaction between DDB1/CRBN and BCL6, the donor andacceptor antibodies are brought into proximity. Excitation of the donorantibody triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665 nm. This specificsignal is proportional to the degree of interaction mediated by ARV-393 betweenDDB1/CRBN and BCL6. This homogeneous assay is simple to perform and requires nowashing steps.



Components

Table 1. Kit Components and Storage Conditions

Component

Concentration

100 TESTS

500 TESTS

2500 TESTS

10000 TESTS

Storage Temperature

Tag1-DDB1/CRBN protein,

50×

10μL

50μL

250μL

1000μL

-80℃

Tag2- BCL6 protein

50×

10μL

50μL

250μL

1000μL

-80℃

ARV-393 (100 μM)

100×

5μL

25μL

125μL

500μL

-80℃

Anti-Tag1 Eu dye antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

20×

25μL

125μL

625μL

2500μL

-80℃

Detection buffer

1×

2ml

10ml

10mL

40mL

-80℃
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Exchange/Return Notes
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SKU: 72171513135

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4.9 ★★★★★
Based on 29 reviews
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Product Reviews
K
Verified Purchase
Kindle Customer Maureen
Lowell, US
★★★★★ 4
Slow, sorry but good
Format: Kindle
This was a slow moving book. Lots of character pov chapters, lots of superfluous descriptions and endless courtly appearances stalled this book to start. Once you get into the heart of the story, it takes off. Before you know it the book is done. My favorite character is Reyna. She is so strong. She is true to herself. She gets into a lot of trouble with her headstrong ways but it's entertaining. I have high hopes for Lorcan. He is honorable to a fault. Thane had turned out to be better than I thought but i still don't like him. Eislin is useless. Great plot twists at the end. I'm looking forward too book 2.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 6, 2020
F
Verified Purchase
Faifre6
Charlottesville, US
★★★★★ 5
Very detailed! Beautiful world building! Strong Heroine!
Format: Kindle
Starts off a little slow and confusing with different POV’s, but starts to all come together towards the middle to make an elaborate plot line and makes it all worth it. Beautiful world building and attention to detail as well as great writing. The cliffhanger was gut wrenching! Can’t wait for the next book!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2020
I
Verified Purchase
Isabelle
Omaha, US
★★★★★ 4
Interesting
Format: Kindle
This was a very captivating book once you got into it thoroughly. But the third person perspective was a bit hard to get used to. But as you got into it and followed the different characters, it was interesting and filled with intrigue, conflict and forbidden love. I can’t wait to read the next one and to complete the series.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 12, 2022
L
Verified Purchase
Lisa B.
New York, US
★★★★★ 5
OUTSTANDING
Format: Kindle
This was very, very good. The world is vast and characters are complex. There is a good plot with a whole lot going on. This is well written. Good twists and turns and some heart breaking moments. You will love these characters, they have heart and loyalty. I am hoping that there will be several more books. We've yet to see anything from the Sea Court but only a mention of them here and there. The Wood Court was given a quick couple of scenes, and only as far as some warriors, we've yet to enter their court and the Shadow Court, I'm not sure if they will be a force for good or bad, but they definitely will play a much bigger role moving forward. This is primarily the Ice and Air Courts. Told in multiple views, which I loved, it gives you a chance to see things from different eyes. There's alot of political maneuvering and deception. I loved it and will pick up the next book as it becomes available. If you like The Fae and the courts, you should love this. I think the author has mucn in store for us.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 8, 2020
J
Verified Purchase
Jessika
Charlottesville, US
★★★★★ 4
Definitely worth the read!
Format: Kindle
After taking a deep breath and taking in that wicked twist of an ending, I have finally composed myself. My first thought when I started this book was that I love Reyna's character. I was intrigued by her connection with her familiar and the Ruin that is plaguing her land. It came as no surprise that she took her sister's place in an attempt to protect her. When they reach the Air Court everything slows down. This is where it was iffy for me. First of all, I like multiple POV's in books however 7 is a bit much. It starts to interrupt the story line. I felt like I was finally making progress connecting with one character, then it was switched to another person. I felt they all had necessary or pertinent information but not necessarily were they all POV worthy. The only other thing that annoyed me was that Reyna constantly was " trapped." She would rush off without thinking, only to need rescuing. She is brilliant in a fight, but she really doesn't think through anything. Lorcan is amazing. I know he might be on the "bad" list, but his background is so interesting. Eislyn(Reyna's sister) is really so sweet, but calculating. I enjoyed her and Thane's dialogue. The author did an amazing job with the imagery in this book. Everything was so detailed it was easy to fall into the scene. I love unexpected twists and while part of the ending I expected, I wasn't expecting how it took place. All in all, I found it very entertaining and I am very invested in continuing this series. Favorite quotes: "The truth may be twisted but never false." "Who was she if she was not the enemy of the Air Court? What was her purpose of she no longer has that?" "In a war-torn land, love was always a lie."
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2020

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