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Description
Human uPAR ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Plasminogen Activator, Urokinase Receptor (uPAR). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Plasminogen Activator, Urokinase Receptor (uPAR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Plasminogen Activator, Urokinase Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The urokinase-type plasminogen activator receptor (uPAR), also known as the urokinase receptor or CD87 (Cluster of Differentiation 87), is a protein encoded by the PLAUR gene in humans. It is a multidomain glycoprotein tethered to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase (also known as uPA) on the cell surface. uPAR is composed of three tandem LU domains, a member of the three-finger protein family. The structure of uPAR in complex with a peptide antagonist and its native ligand, urokinase, has been solved by X-ray crystallography. uPAR also interacts with several other proteins, including vascular proteins, uPAR-associated protein (uPARAP), and the integrin family of membrane proteins. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Smells Like You Have Your Life Together
Scent: Bergamot, Size: 16 Fl Oz (Pack of 1)
Cremo Body Wash with Italian Bergamot, Neroli Blossom, and Fresh Vetiver smells far more expensive than it has any right to. The scent hits that sweet spot between fresh and refined. The bergamot gives it a clean citrus lift, the neroli adds a smooth floral edge, and the vetiver grounds it with a subtle, masculine depth that does not scream but absolutely speaks.
The lather is rich and concentrated, so you only need a small amount. It feels more like something you would find in a boutique hotel than on a regular store shelf. The fragrance lingers just enough to be noticed without overpowering your cologne or filling the entire room.
If you want to step out of the shower smelling like competence and quiet confidence, this one delivers. It is polished without trying too hard, and it makes everyday showers feel slightly upgraded.
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Reviewed in the United States on February 28, 2026
★★★★★ 5
A major upgrade from standard drugstore brands—sophisticated scent & better lather.
I have been a long-time user of standard body washes (think Old Spice, Axe, or Dove Men), but I kept seeing hype about Cremo and decided to see if it was worth the slightly higher price tag. After using the 32oz Spice & Black Vanilla for a week, I can confidently say this is a massive upgrade for your shower routine.
Here is how it stacks up against the typical grocery store brands:
1. The Scent is on another level:
Most cheaper washes smell like generic "Sport" or "Ocean" cologne. This scent is much more complex and earthy.
Top Notes: It starts with a sharp, spicy kick (that's the cardamom).
Finish: It settles into a smooth, warm vanilla and tobacco scent. It smells more like a high-end department store fragrance than a soap.
Staying Power: The scent actually lingers. If you shower in the morning, you will still catch subtle hints of the warm spices for a few hours, which pairs really well with woody colognes.
2. Performance & Feel:
The consistency is thicker than what I'm used to. You only need one solid pump to get a rich lather with a loofah. Crucially, it rinses clean without leaving that "squeaky" residue that dries out your skin, but it also doesn't leave a slick oily film like some ultra-moisturizing washes do. It hits a perfect balance.
3. Value:
The 32oz bottle is substantial. Because the gel is concentrated, I expect this bottle to last 3-4 months easily, making the cost per ounce very reasonable compared to buying smaller bottles of cheaper stuff every few weeks.
Pros:
Sophisticated, "grown-up" scent profile.
High-quality pump (sturdy, doesn't clog).
Non-drying formula is great for colder months.
Cons:
Potency: The scent is strong. If you are sensitive to fragrance or prefer unscented products, this might be overwhelming. The bathroom will smell like it for a good 20 minutes after you're done (which I enjoy, but keep it in mind).
Verdict:
This bridges the gap between basic hygiene and luxury grooming. If you are tired of smelling like "Blue Sport" and want something warmer and more refined for the fall/winter, this is absolutely worth the switch.
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Reviewed in the United States on November 26, 2025
★★★★★ 5
It smells as good as my cologne.
I never go out without a shot of Guerlain L'Homme Idéal L'Intense cologne. Guerlain does not offer a layered line of products with similar scents. I wanted a deodorant and body wash to match. None of the Old Spice scents came close. Axe Oud Wood & Dark Vanilla antiperspirant has more vanilla and is a bit subdued but works.
Finding a body wash was harder. Cremo Spice & Black Vanilla Body Wash sounded promising, but I was apprehensive about its scent including tobacco. I did not want to smell like an ashtray. But when I opened the bottle, I was met with a surprising scent from the past. I immediately recognized it as premium Danish pipe tobacco. Most people do not recognize this aromatic scent as coming from tobacco at all. In fact, it smells more like fresh cured tobacco itself, not its smoke. This adds a very pleasant and masculine smell to the body wash on top of the spice, wood, and vanilla scent I was searching for.
The scent only lasts a few hours after you rinse off. One push on the dispenser puts enough soap on a shower loofah to wash your whole body. I do not rinse off my loofah; I squeeze the soap out of it to wash my face, then I place it back in its tray next to the bottle. The scent of the remaining soap acts like incense until my next shower.
I was also pleasantly surprised to find that this body soap has a rich lather and cleans as well as advertised. I had forgotten the reason I bought the body soap until I used it.
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Reviewed in the United States on February 27, 2026
★★★★★ 5
Intoxicating Smell!
My 'old' shower gel was getting dangerously low prompting a search for a replacement. I looked over recommendations of various sources including Amazon which led me to this Cremo product.
Over the years I've purchased just a handful of products that were either colognes, soaps of various types, or aftershaves, which really stood out for their intoxicating scent. Additionally, they might have had the incredible ability to have a lasting effect of several hours. These products stood out in a way that they also were noticed by the people around me, commenting on the great odor and inquiring as to what it was.
This Cremo product is in that category. The first time I used it clearly put it in that rare product list. Additionally, some of the other qualities of this product that others have commented on I have found to be true. A little goes a long way! You don't need to use nearly as much as some other products that I've had in order to get it to effectively wash body or hair. It truly does leave a soft feeling after using. I didn't realize how big the container would be when I purchased it, however can now say after a few weeks of using it, this is going to last me a long time! And I'm definitely pleased about that. I guess you can say that there are definitely other less-expensive products out there, but when you consider how well this product performs, it's a great buy!
If you're currently reading this, examining products for yourself, wondering if this one is for you....hesitate no longer. When this container runs out....it's definitely going to be a long while....I will definitely buy it again.
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Reviewed in the United States on January 17, 2026
★★★★★ 5
Warm base of vanilla, with a bit of spice to keep things interesting! Definitely a keeper!
First, I need to admit that Cremo is one of my “go to” brands for body wash. I love the fact that the scents are high-quality without getting into the weird “axe” arena of being able to be smelled a mile away.
The body wash has a fresh, warm, earthy smell to it. Vanilla base with a bit of spice to not make it smell like a sugar cookie. I think you all know those body washes that are just too heavy with the vanilla smell. The price for this level of body wash is spot on for what you get from a quality, moisturizing, and skin care perspective. It doesn’t dry out your skin and even the deep rich color of the gel screams quality.
This is a really great new scent from Cremo and one that I’d definitely come back to and keep in my rotation.
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Reviewed in the United States on April 25, 2026
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