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Description
Human GAL10 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a galectin-10 (GAL10) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of galectin-10 (GAL10) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human galectin-10（GAL10) ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Galectin-10 acts on biological membranes to regulate multifunctional lysophospholipids. It is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine into glycerophosphatidylcholine and a free fatty acid. Galectin-10 may have carbohydrate-binding or IgE-binding activity. It is structurally and functionally related to the galectin family of β-galactoside-binding proteins. Galectin-10 may be involved in inflammation and some myeloid leukemias. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.8 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
So cute! Durable!
Size: 1 Count (Pack of 1), Style: Shark
These are my dogs “babies” and we are on #3! Adorable! She loves them!
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Reviewed in the United States on May 25, 2026
★★★★★ 5
great dog toy for small and large!
Size: Medium (Pack of 1), Style: Gator
we have a very large silver lab that tears through toys, and very fast! some toys only last a day while others last a week or so... he has yet to tear this on up at all and we have had it for about 3 weeks. we also have a very small teacup yorkie, so some toys we get he cannot even play with, this one he can squeak and carry around which is great! it very quickly became one of their favorite toys! will buy again if he ever does destroy it !!!
update: after a month of the lab and small yorkie playing with this some of the fabric stared to tear on the end of the toy, they still love it and it still squeaks perfectly fine. didn't think it would last this long with the big lab, will order new one when they do finally destroy it for good
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Reviewed in the United States on September 10, 2015
★★★★★ 4
A must have for my little puppers
Size: Small (Pack of 1), Style: Monkey
Bought this for a Chihuahua. He came from a bad situation that gave him screaming dreams of terror in the middle of the night, waking up, screaming. I would find him frozen in absolute terror. Poor dude. Now, he seems full of rage, and I get it because I know half of his story. And we love him anyway. He has 2 big brothers that will help show him the way, living his best life ever!
Long story short; I first started buying this Petstages toy many years ago for my blind MinPin. Seeing it still, out here for sale always puts a smile on my face. Back then, they were a better quality. Now-a-days, the material around the squeaky is on the loose side, making it easier to destroy when my big dogs get it.
Still, it brings back wonderful memories, is soft for a small dog that doesn’t destroy his things, it squeaks and they are usually loud (which is a must have, if you ask me), and it is cute to boot!
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Reviewed in the United States on April 12, 2025
★★★★★ 5
THE favorite toy
Size: 1 Count (Pack of 1), Style: Shark, Size: 1 Count (Pack of 1), Style: Shark
I found this toy used at a thrift store almost 1 year ago. My Shih tzu mix loved it so much till all the seams ripped out and 2 out of 3 squeekers fell out and it was shambles, hardly usable at all. 1 week ago I decided to try my luck to find a similar shark toy for Finnegan. I couldn't believe my eyes when I found THE EXACT same toy!! When I unboxed it, I put the old and new side-by-side and imagine his surprise, it was priceless! Very pleased with this.
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Reviewed in the United States on May 6, 2026
★★★★★ 5
Best Dog Toy Ever!
Size: Small (Pack of 1), Style: Monkey, Size: Small (Pack of 1), Style: Monkey
Unbelievable. See below previous reviews. Today is 3/22/2023 (4th Update): Cavachon is now three years old. THIS MONKEY is STILL played with DAILY. She’s played with and lost interest in dozens of other toys in the past three years, but Monkey is always the go-to. Now only on Monkey #6, which is remarkable considering the daily torture she puts Monkey through. Other toys get destroyed, but Monkey endures. This toy is our dog’s best friend and playmate!
10/9/21 (3rd update): Its rare that any toy (without food inside) keeps the interest of our Cavachon for even 30 minutes… yet day after day, she runs around with Monkey for at least an hour. She’s on her 4th Monkey… and this thing gets mauled, chewed, super-squeaked, fetched, and shook violently EVERY DAY. Durability times ten!
2/21/21 (2nd Update): Crazy pup is now 14 months old, and she plays DAILY with “monkey”! She’s on her third monkey because eventually she pulls off the tail, but each of her first two lasted months.
ORIGINAL REVIEW MAY 2020: There is something really special about this monkey... Our 5-month-old Cavachon LOVES this toy. I recently ordered a second one, in case she wears out the first one. She knows it’s her “monkey” and finds it when we ask her “Where’s monkey?” She fetches it, walks around squeaking it, and she also zooms around the house shaking it furiously by the tail. She’s been playing with, and chewing on all parts of It, DAILY for three months. In the photo, you can compare the brand-new one, still on the cardboard, with the one she’s mauled for three months. Not much difference. I would say this is an extremely durable, and safe, toy.
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Reviewed in the United States on May 15, 2020