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Description
Alexa Fluor® 488 Mouse Anti-Human CD3d Antibody (S-R565)Product Specification Host Mouse Antigen CD3d Synonyms T cell surface glycoprotein CD3 delta chain; T cell receptor T3 delta chain; T3D; CD3D Location Cell membrane Accession P04234 Clone Number S R565 Antibody Type Mouse mAb Isotype IgG2a Application FCM Reactivity Hu Positive Sample Human peripheral blood leukocytes Purification Protein A Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0.
Product Specification
| Host | Mouse |
| Antigen | CD3d |
| Synonyms | T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3D; CD3D |
| Location | Cell membrane |
| Accession | P04234 |
| Clone Number | S-R565 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood leukocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD3d (CD3-delta) is a 19 kDa transmembrane glycoprotein that together with CD3ε, CD3γ and CD3ζ forms the CD3 signaling complex, which is tightly associated with the T-cell receptor (TCR) on thymocytes and mature T lymphocytes; upon antigen/MHC engagement the CD3 chains become phosphorylated on their cytoplasmic ITAMs by Lck and Fyn, initiating the cascade that leads to T-cell activation, proliferation and differentiation, while during thymic development CD3d is indispensable for correct assembly and surface expression of the pre-TCR/TCR complex and thus for positive selection of CD4⁺ and CD8⁺ T cells.
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