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Description
Rat ARNTL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Aryl hydrocarbon receptor nuclear translocator-like protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aryl hydrocarbon receptor nuclear translocator-like protein, also known as ARNTL or brain and muscle ARNT-like protein 1 (BMAL1), is a protein encoded by the BMAL1 gene. BMAL1 encodes a transcription factor with a basic helix-loop-helix (bHLH) and two PAS domains. This 62-amino acid protein plays a key role as one of the positive elements in the mammalian autoregulatory transcription-translation negative feedback loop (TTFL), which is responsible for generating molecular circadian rhythms. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissuehomogenatesandotherbiologicalfluids |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Fun and Educational | We Read It All the Time!
Format: Board book, Format: Board book
This is one of our most read books right now! My toddler absolutely loves it, and it’s been such a fun way to start learning about the planets. The illustrations are engaging and it keeps her attention the whole time.
I’ve noticed she’s actually starting to recognize and name some of the planets, which is amazing at her age. It’s the perfect mix of fun and educational.
Definitely a great book if you’re looking to introduce your little one to space in a simple and engaging way!
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Reviewed in the United States on April 29, 2026
★★★★★ 5
Engaging and Educational! Perfect for Little Astronomers! 🌟
Format: Board book
"8 Little Planets" is an absolute delight for young readers and their parents alike! As a parent of a toddler who is just starting to explore the wonders of space, I found this book to be a fantastic introduction to the solar system. The colorful illustrations are vibrant and captivating, immediately drawing in little ones' attention. The rhyming text is fun and easy to read aloud, making it a joy to share this book during storytime.
What I love most about "8 Little Planets" is how it manages to make learning about the planets so engaging. Each planet is given its moment to shine with a little story that is both informative and age-appropriate. The book also subtly incorporates the latest astronomical knowledge, like the reclassification of Pluto, which is a great touch.
It's clear that this book was created with care and attention to detail, and it's become a favorite in our home. Whether you're raising a future astronaut or just looking for a fun way to introduce your child to science, "8 Little Planets" is a stellar choice!
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Reviewed in the United States on August 22, 2024
★★★★★ 5
Solar system board book:EXCELLENT, INFORMATIONAL AND FUN
Format: Board book
My niece loved this book. As did I! I always found outer space fascinating, but have forgotten many facts from my youth. When I ordered it, read it through and was very happy about all the information packed I. Such a fun book. There is even an honorary mention of Pluto. Vibrant, fun, factual, EXCELLENT! she got a astronaut costume also, so now she’s got all the tools for her imaginary space travel.
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Reviewed in the United States on February 20, 2026
★★★★★ 5
Great book for your little ones!
Format: Board book
One of the faccoutite book of our 2 year old - the pictures are great, and the fact for each planet is quite interesting.
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Reviewed in the United States on April 16, 2026
★★★★★ 5
Our favorite book
Format: Board book
This is our favorite book to read. Its got a nice rhyming flow, its not too long, its fun information and the illustrations are adorable! We've used it so much we may need to buy another soon because of the wear and tear
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Reviewed in the United States on April 13, 2026