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Description
Rat TGF-b3 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Transforming Growth Factor Beta 3 (TGF-b3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta 3 (TGF-b3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Transforming Growth Factor Beta 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming growth factor β3 (TGF-β3) is a protein encoded by the TGFB3 gene. It is a cytokine involved in cell differentiation, embryogenesis, and development. It belongs to the large family of cytokines within the transforming growth factor β superfamily, which includes the TGF-β family, bone morphogenetic proteins, growth and differentiation factors, inhibins, and activins. During palate development, it regulates molecules involved in cell adhesion and extracellular matrix formation. Without it, mammals develop a malformation known as cleft palate. This is caused by the failure of epithelial cells on either side of the developing palate to fuse. It also plays an important role in controlling lung development in mammals by regulating cell adhesion and extracellular matrix formation in this tissue. It also controls wound healing by regulating the movement of epidermal and dermal cells in injured skin. It interacts with TGF-β receptor 2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
Practical, logical, and comprehensive guide to starting and running an Airbnb
Format: Kindle
This is a great resource for anyone who wants to own and operate an Airbnb (short-term rental housing). The book is comprehensive. It starts with the basics and goes on and on, covering every conceivable issue, from startup basics to day-to-day operations, legal issues that might come up, how to hire a cleaning crew—even real estate issues many people might not be aware of. The only thing I found missing was instructions for how to deal with bedbugs, which are a growing problem throughout the world (and can wreak havoc on any rental property).
The book is well-organized, which makes it easy to read… perfect for anyone trying to learn the business from the ground up. It is well-researched, with many examples to show the reader how things work in the real world. There are a number of resources mentioned also: websites for additional information on how to compare rates, and so on.
The author is practical and pragmatic. He balances caution with enthusiasm (and vice versa). I recommend it to anyone who wants to gain additional income through Airbnb.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 14, 2024
★★★★★ 5
What a Great Book!
Format: Kindle, Format: Kindle
"Starting a Successful Airbnb Business for Beginners" is a must-read guide for anyone who has ever considered entering the short-term rental market. The author's writing style is clear and concise, making it easy to follow the step-by-step approach to creating a profitable Airbnb business. This book is perfect for beginners who are just starting their journey and are unsure of what to do next. The author provides valuable insights into how to go from 0 to your first property and how to make it "guest ready." The book also covers essential topics such as setting up a budget, finding an ideal property location, listing your property, and achieving Superhost status.
One of the standout features of this book is its practicality. The author provides a wealth of tools and information that can be easily applied to create a successful Airbnb business. The action steps at the end of each chapter are particularly motivating and provide readers with a clear roadmap for moving forward. Moreover, the book's emphasis on creating a budget and understanding associated costs ensures that readers are prepared for the financial aspects of running an Airbnb business. Overall, "Starting a Successful Airbnb Business for Beginners" is a highly recommended read for anyone who wants to tap into the $1.2 trillion short-term rental market and earn extra income.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 4, 2023
★★★★★ 5
Will help you to start your Business
Format: Kindle
Starting a Successful Airbnb Business for Beginners is a great guide that will help you to start your short-term rental business. It will give you tips and basic infos on how the business works and will suggest sites and Apps that are useful for you.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 30, 2025
★★★★★ 5
Amazing, different, success.
Format: Kindle
First let me say that this book (series) was not something I normally read. I prefer LGBT books written by women, but I also had no idea what a LitRPG was.
I started the book cautiously, I found myself a little disappointed that it was under LGBT but the main character was into her co-worker. The co-worker was a dude. But I kept reading and I was surprised by how I got into the fact that it was sending her into a realm where she was basically a character in a video game. Completing tasks to earn points, leveling up, gaining stats.
Now I’ll be honest, some of the information in this book (and the rest of the series) flew straight over my head. The author is definitely far more intelligent than I, and probably most average people lol.
Finally, the characters are actually well developed and have their own unique personalities. With Emma’s leadership qualities she helps her group of new friends survive and turn into badasses. In the following books you can see how they each change and take on different roles over the course of their trials.
This first book was great, it got me hooked. But it gets even better as time goes on! I have read this series so many times that I basically know it by heart. It’s that good.
I recommend you read this, and the 5 other novels in the series. Also, check out the authors website and Patreon for sure. He’s currently working on a new series and posts chapter updates in Patreon posts.
PS. I only leave reviews on books that wow me. :)
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 6, 2025
★★★★★ 4
Comes Off Naturally
Format: Kindle
Unnatural Laws introduces a few wrinkles to the isekai LitRPG template and makes a comfy niche for itself.
First, as the title implies, it introduces a few variations to the laws of physics. Water has higher self-cohesion, breathing can be weaponized, gravity's parameters work differently, etc.
Second, the book includes the "forced participation in a training/puzzle facility" subgenre. Instead of a short tutorial arc, the first book ends well before winning free of the deadly training scenarios.
Third, the "portal" mechanism by which folks are yoinked into a new reality. It is implied that the "kidnapped to another world" setup will continue to be important to the plot, where in most other Isekai it doesn't particularly matter how or why the MC got yoinked...it only matters that it happened.
The most important things to me are characters. And these characters work! The MC is snarky, tough-minded, and takes charge. In a romance novel dust jacket, that description would already have me nauseous in anticipation of a vapid young woman who "zings" when silence would serve and who only appears "tough" and "take charge" because all of the other characters react to her that way. Not in this book! In this story, it's real and it's working for me.
The secondary characters are also real to me. A young woman who low-key flirts with everyone (unconsciously?) and gets preferential treatment. An earnest young man who flees from anything he considers "work"...but will happily pour himself into crafts he enjoys. And others that I can only encapsulate even worse, so I won't try! There is plenty going on and plenty more to uncover. I thoroughly enjoy watching them interact and cooperate to navigate their new circumstances.
Other things that are present and well-done, even if they don't stand out quite as much:
- An assistant AI with secrets to keep
- An interesting LitRPG system, blended with cultivation
- A teaser for a well-built new world
- A germinating romantic relationship
If you enjoy the Isekai genre, the only thing that should make you pause before buying this: It's light on combat. Plenty of action, mind you, but most of it isn't combat.
If that doesn't bother you (as it doesn't bother me), then you should pick this up.
=====
EDIT: After reading 5 books, I like this series even better. At this point, I'd revise the subgenre to be "death games" and mention that the death games don't finish until the very end of book 4.
The "light on combat" label expires with book one...there's plenty of combat after that. It is both well done and inventive!
Lastly, I suppose this could be classified as YA fiction. Political landscapes and broader implications are hinted at, but you rarely get a look at them before they arrive and change the landscape. That said, I'm no spring chicken and I enjoyed these books a great deal.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2023
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