SKU: 64689170607

Unione ®️ TR-FRET Human PD1/PD-L1 Binding Kit

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Description

Unione ®️ TR-FRET Human PD1/PD-L1 Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit utilizes homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between PD1 and PD L1. This method enables simple, rapid, and high throughput screening of inhibitors and antibody blockers. As shown in the figure, the interaction between PD1 and PD L1 is detected using an Eu labeled anti Tag1 antibody (TR FRET donor) and an Ac labeled anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.

As shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.

Components

Component

Concentration

100T

500T

2500T

10000T

Storage Temperature

Pembrolizumab

5μM

5μL

10μL

50μL

200μL

-80℃

Tag1-PD-L1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Tag2- PD1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃


Note: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
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  • To process your return/exchange, please contact us at [email protected]
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SKU: 64689170607

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4.7 ★★★★★
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eff me
Chelsea, US
★★★★★ 3
great for what im doing. maybe not as good in a vehicle though
Size: 36 sqft
decent product. not as thick as typical sound deadening but definitely cheaper. and for my application, i didnt need a name brand thicker material. i am putting it on our heat and ac vent and intake pipes during our remodel. sound travels from room to room in our old house through these vents and pipes so a little sound deadening goes a long way
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Reviewed in the United States on June 2, 2026
M
Verified Purchase
Miguel
Houston, US
★★★★★ 5
Quality
Size: 36 sqft
Good item
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Reviewed in the United States on May 30, 2026
A
Verified Purchase
Amazon Customer
Natrona Heights, US
★★★★★ 2
Buy the individual cross cut sheets, not these rolls.
Size: 36 sqft
I ordered two different types, individually cross cut foil rectangular pieces and these rolls both containing 36. sq. ft. The cross cut sheet blows this away. This stuff is much thinner, much less butyl rubber, much flimsier foil, and needs twice as much to have the same effect. Once you put it on, you cant remove it. The other brand's 2mm is much thicker than this.
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Reviewed in the United States on June 3, 2026
B
Verified Purchase
Bradley
Lexington, US
★★★★★ 5
Excellent product high quality.
Size: 36 sqft, Size: 36 sqft
This stuff definitely suprised how easy it was to work with. Not to mention it sticks very well. Don't put it on anything you may need to get back into. It will take the paint off of a service area. I have a 2018 dodge durango. I just had a aftermarket audio sytem installed. It was rattling so bad it was driving me crazy. I seen this stuff and decided to give it a try. Its a night and day difference no moore rattling. I box will be enough to do my back gate and all front and rear doors also. For the price you honestly can not beat it. If your on the fence about trying it don't be. You definitely won't regret getting it. Its worth every penny. You don't realize how much something rattling in your vehicle robs you of spund quality. Glad I made the decision. Now off to the other 4 doors. ,and then the roof. One of the things I really like was the fact it has multiple line to help you make even better cuts. Definitely will buy a gen.
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Reviewed in the United States on February 28, 2026
M
Verified Purchase
Mr. & Mrs. Us
Bozeman, US
★★★★★ 5
Affordable, Clean and does the job.
Size: 36 sqft, Size: 36 sqft
I consumed about 90% of this box doing all doors and the tailgate on a 2000 tahoe. I applied it very liberally inside every door (exteror) panel. We then added an almost equal amount of deadening/absorbing/decoupling foam to the other side of the door (panel side). If you know how many rattles a circa 2000 tahoe GMT800 can have by now, you can appreciate why I added this as a "must" if i were to put some money back into this vehicle. Best sub $150 spent on this mini resto so far, right next to tint. Good quality. Not to thick or thin. Good sized sheets. Seamed to deaden as good as another slightly thicker material i recently used in my F250. My box was delivered with no issues of melting or anything funky. Clean, no major smell before or after and easy to work with. Maybe buy a roller set and wear gloves. Save your hands if your doing inside of panels. Good buy. Will buy more for future projects.
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Reviewed in the United States on February 17, 2026

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