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Description
Human MAPK13 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Mitogen Activated Protein Kinase 13 (MAPK13). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Mitogen Activated Protein Kinase 13 (MAPK13) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Mitogen Activated Protein Kinase 13 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Mitogen-activated protein kinase 13 (MAPK13), also known as stress-activated protein kinase 4, is an enzyme encoded by the MAPK13 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases serve as integration points for multiple biochemical signals and are involved in various cellular processes, such as proliferation, differentiation, transcriptional regulation, and development. This kinase is closely related to p38 MAP kinase, and both can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3 and 6 can phosphorylate and activate this kinase. The transcription factor ATF2 and the microtubule dynamics regulator stathmin have been shown to be substrates of this kinase. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
Great cleaner
Great great! Cleans perfectly and easy to use, just drop it in the bottle washer. Super time saving and great value.
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Reviewed in the United States on March 25, 2026
★★★★★ 5
A Game-Changer for Breastfeeding Moms!
I was a bit skeptical about buying a specialized bottle washing block at first, but I'm so glad I took the plunge! The Momcozy Official Washing Block is a total game-changer for breastfeeding moms like myself. This product has made my life as a nursing mom so much easier and more convenient.
First of all, let me just say that this block is SUPER easy to use. It's simple to pop in a dirty bottle into the machine, and before you know it, your bottles are sparkling clean and ready for reuse. The block itself is also very compact and lightweight, making it easy to store in my nursing bag or diaper bag.
But what really sets this product apart is its ability to get out even the toughest stains and residue. I've tried other bottle washing methods before, but nothing compares to the effectiveness of this block. My bottles come out looking like new every time!
The best part? The Momcozy Official Washing Block is affordable and makes a great investment for any breastfeeding mom. Trust me when I say that you won't regret buying this product!
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Reviewed in the United States on December 27, 2025
★★★★★ 5
Amazing
The Momcozy Bottle Washer Tablets have been such a great addition to our daily routine. As a busy parent, anything that makes cleaning bottles easier is a huge win, and these tablets really do the job well.
They dissolve quickly and help remove milk residue and buildup from bottles, pump parts, and other baby feeding accessories. After using them, everything comes out clean, fresh, and residue-free, which gives me peace of mind knowing my baby’s items are properly sanitized.
I also love how convenient they are. Instead of measuring or dealing with messy liquids, you simply drop a tablet in and let it work. It saves time and makes the whole process much simpler, especially during those busy days and late-night bottle cleanups.
Another plus is that they seem gentle but effective, which is important when cleaning items used for babies.
Overall, these tablets are easy to use, effective, and a real time saver. I would definitely recommend them to other parents looking for a simple and reliable way to keep baby bottles and feeding supplies clean. I will absolutely be purchasing them again! ✨
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Reviewed in the United States on March 7, 2026
★★★★★ 5
clean well
These work great in the Momcozy bottle washer. They are small and easily fit in to the center spot of the top rack. I occasionally find residue left on the bottles or nipples, but it is usually an indication I need to run a clean cycle on the machine and not due to the washer tabs themselves.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
An actual towel for your face and body!
Size: 50 Count (Pack of 1)
These are the most durable and portable hand towels ever. The material is not cotton but it is soft and plush enough to not feel rough against your face or hands or body.
The size of the towel, once it is wet, it is a little bigger than the size of a washcloth for the body.
So easy to use- wet the towel with water and use for your body. A great buy indeed!! Highly recommended!
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Reviewed in the United States on March 31, 2026