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Description
Rat CAPN2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a calpain 2 (CAPN2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of calpain 2 (CAPN2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Calpain 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Calpain 2 (Calpain2) is a protein encoded by the CAPN2 gene. Calpains are calcium-activated neutral proteases and non-lysosomal, intracellular cysteine proteases. This ubiquitous enzyme consists of heterogeneous forms with different large catalytic subunits associated with a common small regulatory subunit. The gene encoding the large subunit of the ubiquitous enzyme, calpain 2, has multiple heterogeneous transcription start sites in its 5' UTR. It has been shown to interact with Bcl-2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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★★★★★ 5
Pigeons don't eat it as much. Good thing
Style: Wild Bird Food
They love it
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Reviewed in the United States on May 18, 2026
★★★★★ 5
Great filter at Great price.
Pattern Name: 14x14x1, Style: Merv 8
Prefect fit and works great. We have these on subscription to match our system filter replacement reminder.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
⭐️⭐️⭐️⭐️⭐️ I can breathe again — and my house says thank you 😂
Pattern Name: 20x20x1, Style: Merv 11
⭐️⭐️⭐️⭐️⭐️ I can breathe again — and my house says thank you 😂
These Amazon Basics MERV 11 air filters have been a game changer for my HVAC system. I installed them and immediately felt like my home went from “dust tornado aftermath” to “spa-day air vibes.” Whether it’s pollen, pet hair, or mystery dust bunnies lingering in the corners, these filters make the air feel noticeably cleaner and fresher.
Installation was about as easy as ripping plastic wrap off a new gadget — pop out the old filter, slide these in, and suddenly the air quality goes from “huh?” to “ahhh.” The electrostatic pleated design grabs everything from sneaky dust motes to pet dander like a champ, and I can actually tell when the AC kicks on that the air feels crisper — in a good way. 🌬️
Between the six count and the solid MERV rating, this was a smart purchase that keeps my HVAC happy and my allergies way more manageable. If you want clean air without the hassle, these filters are a fantastic value and really get the job done. Highly recommend!
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Reviewed in the United States on February 26, 2026
★★★★★ 5
Amazon Basics MERV 11 Filters: Solid Performance Without the Premium Price
Pattern Name: 20x20x1, Style: Merv 11
Amazon Basics MERV 11 Filters: Solid Performance Without the Premium Price
I’ve been using these Amazon Basics 20x20x1 MERV 11 filters in my home HVAC system, and overall they’ve been a very dependable option for routine filter changes. I like buying them in a 6-pack because it takes the hassle out of remembering to reorder every couple of months, and the per-filter cost is noticeably lower than the big-name brands at the hardware store.
Installation is straightforward. The actual size fits my return slot properly with no bending or forcing, and airflow has remained strong. With MERV 11, these strike a nice balance for my setup — noticeably better dust and pollen capture than basic MERV 5 or 8 filters, but without putting obvious strain on the system. After about a month of use, the filters clearly show they’re doing their job, collecting a visible layer of dust and fine debris.
A few things to keep in mind. The cardboard frame isn’t as rigid as some premium filters, and they’re a bit thinner than brands like Filtrete. They’ve held up fine for me during normal use, but I wouldn’t try to stretch them much past the recommended replacement interval. Also, if you have an older or low-powered HVAC system, MERV 11 may be more filtration than you need — in that case, a lower MERV option might make more sense.
Bottom line: These Amazon Basics MERV 11 filters are a practical, budget-friendly choice that deliver solid filtration and good airflow for regular HVAC maintenance, as long as you stick to timely replacements and don’t expect premium-brand rigidity.
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Reviewed in the United States on January 4, 2026
★★★★★ 5
Perfect fit, lasts a long time, and a great price.
Pattern Name: 20x20x1, Style: Merv 5
These filters are a perfect size, they're well-made (I don't notice any more dust in the house than "normal"), and so far, they last longer than I had expected (they're made to last 30 days, and the one I have in has lasted 90 days). So for this 6-pack, what should have lasted 6 months may actually last more like 3 years! And these are the best deal I could find on the internet. I'd definitely recommend them.
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Reviewed in the United States on April 12, 2026