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Description
Human DMT1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against natural resistance-associated macrophage protein 2 (DMT1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of natural resistance-associated macrophage protein 2 (DMT1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Natural resistance-associated macrophage protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Divalent metal transporter 1 (DMT1), also known as divalent cation transporter 1 (DCT1) or natural resistance-associated macrophage protein 2 (NRAMP 2), is a protein encoded by the SLC11A2 (solute carrier family 11, member 2) gene. It represents a large family of orthologous metal ion transporters. As its name suggests, it binds a variety of divalent metals, including cadmium, copper, and zinc. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
An Actual Nice Chair for a Decent Price. +2 for the Tool.
Color: Black Grey
Welp, My old chair finally broke, so It was time for a new one. I ended up coming across this chair here, and I couldn't really find anyone talking about it online. I decided to give it a shot since it was on a good sale for Christmas, going down to 99$. I'm going to be honest, I am pleasantly surprised. The cushioning for it is a little firm, but that is perfect for me. Its pretty average when it comes to office chairs, but having the added lower back support is a great touch. I have no complaints about it.
The one thing that I am actually really happy about isn't on the chair. Don't get me wrong, over the week I have been using it, It has been nothing but great. That thing is the actual tool it came with. Its a basic Allen key, but the top of it is a molded handle instead of a "L" piece of metal. It made the already easy process of putting together this chair even better. Even after this chair has ran its course like all other chairs, I'm going to still use this tool.
So yeah, I think 5 - stars pretty accurately describes how I feel about my chair.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 2, 2026
★★★★★ 5
Comfortable chair that works great for long hours
Color: Black
I’ve been using this office chair for a few days and it’s been very comfortable for working and longer desk sessions. The high back design and ergonomic support help maintain good posture, while the cushioning stays comfortable throughout the day. The chair feels sturdy and stable, and the 330 lb capacity gives it a solid, durable feel. It also rolls smoothly and quietly, which is nice when moving around the workspace without creating a lot of noise. Overall, it was easy to assemble, looks professional, and offers great value for the money.
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Reviewed in the United States on June 10, 2026
★★★★★ 5
Very Comfortable Ergonomic Chair - Great for Long Work Days!
Color: Black, Color: Black
I’ve been using this ergonomic office chair for a couple of weeks now and I’m genuinely impressed. For the price, it offers excellent comfort and support.
What I like:
• Very comfortable padded seat and backrest — I can sit for 8+ hours without back pain.
• Good lumbar support and adjustable height/armrests.
• Holds up to 330 lbs easily (I’m 240 lbs and it feels very stable).
• Smooth rolling wheels and sturdy base.
• Easy to assemble (took about 20 minutes).
The mesh back breathes well and doesn’t get hot. It looks professional in my home office too.
Overall, this is a solid ergonomic chair. If you spend a lot of time at a desk, I highly recommend it.
Would I buy it again? Yes, definitely.
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Reviewed in the United States on May 12, 2026
★★★★★ 4
Great if you're average height.
Color: Black
Comfortable for the price. The seat could be a bit better, but for a budget friendly ergonomic chair, it's well made and has helped a lot with my back pain caused by my previous desk chair.
Assembly wasn't terribly difficult, though I did have one screw with damaged threads. Luckily they ship spare screws in the kit, so that was a non-issue.
Rollers work great on my small rug and hard flooring and don't tend to tangle cords or cables too much.
Size wise, works great for my frame (5 ft 6 in) but for someone more than a few inches taller than myself, you should probably look at another chair.
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Reviewed in the United States on September 4, 2025
★★★★★ 5
Great chair
Color: Black
I am very happy with my purchase. Easy to assemble but be aware your drill bits included with your set won't fit their screws. However, you can get a fair bit done with the drill and then use tool included to adjust. Super comfortable and exactly the right height for my desk. Highly recommend for quality exceeding price point.
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Reviewed in the United States on May 9, 2026