Pay in installments of $51.59 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 28 - Aug 2
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse ALK ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a serine/threonine-protein kinase receptor R3 (ALK) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of serine/threonine-protein kinase receptor R3 (ALK) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Serine/threonine-protein kinase receptor R3 | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Activin receptor-like kinase (ALK), also known as serine/threonine protein kinase receptor R3 or ACVRL1, is an enzyme encoded by the ACVRL1 gene. It is a receptor in the TGF-beta signaling pathway. This gene encodes a type I cell surface receptor of the TGF-beta ligand superfamily. It shares a high degree of similarity with other type I receptors in its serine-threonine kinase subdomain, a glycine- and serine-rich region preceding the kinase domain (called the GS domain), and a short C-terminal tail. The encoded protein shares a similar domain structure with other closely related ALK or activin receptor-like kinase proteins, which form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia (HHT) type 2, also known as Rendu-Osler-Weber syndrome 2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 13 reviews
Sort
Product Reviews
★★★★★ 5
Very happy with my purchase
Color: Black8017, Material Type: Leather
Very high-quality. Holds all my cards in cash.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 6, 2026
★★★★★ 5
Amazing Wallet!
This is a great wallet. I searched through many styles with similar features, but most did not have the pull down side lever to lift the cards. That feature is exactly why I chose this one.
The leather feels high quality and not cheap at all. It is very durable. The money clip is extremely tight and holds cash securely with no risk of anything slipping out. I also wanted a wallet that still opens to display a driver’s license and daily use cards while remaining slim, and this one checks every box.
It is compact but holds a surprising amount. My husband absolutely loves it and he is very picky about wallets. There are multiple slots not only for cards but also for holding papers or notes. There is even an additional slot behind the money clip for extra storage.
The pull down button smoothly lifts all the cards at once and fans them out so each card is visible and easy to grab. I was unsure how this mechanism would work at first, but the cards are slightly staggered just enough to clearly see each one. You simply grab the card you want and push the stack back down into the wallet.
We chose the carbon black version and it is sleek, lightweight, and looks very sharp. My husband mentioned it needs a little breaking in due to the quality construction, but it is still easy to access everything he needs and stays compact, which was most important to him.
It also comes in a very nice black gift box with fitted foam that securely holds the wallet, making it perfect for gifting or safe storage before use.
I highly recommend this wallet. For the price, it cannot be beaten. I searched extensively for the best option at a fair price point, and this one easily compares to wallets that cost close to or even over $100.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 10, 2026
★★★★★ 5
Typecase Wallet for Men
Color: Carbon Fiber Black, Style: Standard Wallet
This wallet has plenty of room for cards and id's. Not bulky nor is it difficult to remove and use cards. Love the appearance and RFID protection. Also love the money clip.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
★★★★★ 5
Great quality wallet
Color: Carbon Fiber Black, Style: Standard Wallet
Holds 6 cards in pop up slot. Others can fit inside the wallet. ID is semi easy to remove. Great quality and durability. Cash doesn’t come loose even in your pocket all day. Will not mag to your phone just fyi.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 28, 2026
★★★★★ 4
Almost Perfect - Compact, Strong Magnet, Minor Card Access Issue
Color: Alaska Black, Style: Standard Wallet, Color: Alaska Black, Style: Standard Wallet
This is hands down the best wallet I’ve ever had. I love how compact it is while still holding quite a bit. On the front, it holds two cards, although I find the thumb slot a little short — it doesn’t extend the second card enough, so you have to dig a bit to get it out. The inside card holder (the non-window one) is also a bit tight.
The magnet that holds the bifold together is very strong. The first section of the wallet (the bifold) holds three cards — two outside with the thumb groove and one inside. I have metal cards in all three slots, so it adds a little extra weight, but the magnet still holds firmly and stays aligned.
The main section has a window for IDs. I keep two IDs and my insurance card there, and even with it being packed, the magnet still holds strong. One small downside: the window isn’t deep enough to fully show my ID, cutting off the date of birth at the bottom. I have to push my license up a bit, which looks a little goofy, but since I rarely need my ID, it’s not a big issue. When I do need it, I can at least show it without taking it out.
The main compartment easily holds 6–8 cards. I’m currently maxed out at 7 cards, including 2 metal ones, and they all have flat surfaces. The push-up button slides smoothly even with the extra weight, and all the cards pop up as they should.
The money clip is very secure too. I haven’t fully tested its limits, but it held a single bill in place without any issues, and right now I have 10 folded bills in there with no problem.
Overall: I love this wallet. It’s compact, easy to access, and for the most part, the cards are very easy to grab.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 25, 2025
recommand products
Vivid Norrköping, Sweden - a paper cut of a city with trees and buildings
24.00
07-09 Jaguar X150 XK XKR Automatic Transmission Floor Gear Shifter w/ Knob OEM
155.95
10-19 Jaguar XJ XJL X351 Engine Battery Starter Motor Negative Wire Cable OEM
41.50
Samsung 34" S6 5VC Curved WQHD Webcam Business Monitor | LS34C650VAMXUE
1010.50
Vivid Olbia, Italy - a paper cut of a colorful city with a boat
19.00