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Description
Human FUT2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against galactoside 2-alpha-L-fucosyltransferase 2 (FUT2). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of galactoside 2-alpha-L-fucosyltransferase 2 (FUT2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Galactoside 2-alpha-L-fucosyltransferase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Fucosyltransferase 2, also known as FUT2, is an enzyme encoded by the FUT2 gene. FUT2 is a phospholipid peroxidase that protects cells from membrane lipid peroxidation. The antioxidant enzyme fucosyltransferase 2 (FUT2) belongs to the glutathione peroxidase family, which consists of eight known mammalian isoenzymes (GPX1-8). FUT2 catalyzes the reduction of hydrogen peroxide, organic hydroperoxides, and lipid peroxides at the expense of reduced glutathione and plays a role in protecting cells from oxidative stress. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
Great for the storm shelter!
Style: Updated 3.46in Magnet L Red Hooks 2PCS, Style: Updated 3.46in Magnet L Red Hooks 2PCS
In our storm shelter we had some lights that we tried to hang up, multiple times, with no luck! Then I found these garage hooks. They are overkill for the lights, but I’m so happy that we now have lighting in the storm shelter. I could think of so many other uses for these. They strength of the magnets is awesome!
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Reviewed in the United States on May 19, 2026
★★★★★ 4
Magnet is super strong but hooks are not adjustable
Style: Updated 3.46in Magnet L Red Hooks 2PCS, Style: Updated 3.46in Magnet L Red Hooks 2PCS
I ordered this set of Mutuactor magnetic hooks because I was tired of thinking I had things organized and then finding previously hung magnets have slid down the wall.
It was a first for me to have to assemble a magnetic hook. This was made slightly more difficult by requiring a wrench. So many items these days arrive with a disposable little hex wrench or whatever is needed for assembly. Not in this case. Maybe the manufacturer assumes if you are spending $15 per magnet to hang your tools you have a wrench handy. Anyway, I got my wrench and found no instructions. I know from life that the order is bolt-washer-lock washer-nut. The next question arose when I found one bolt and several holes... hmmmm. It looks like they wanted to provide the option to screw the hooks directly to the wall. I am not sure the average person would pay that price just for a hook, though. I chose a hole and it seems to be the right one.
The good news is I was quickly in business. Soon one magnet was snatched by my husband for his rolling tool cart. He hung his (very heavy) Sawzall and the magnet never moved. He liked the rubber coating that protects his tools. I chose to take advantage of the amazing grip of this thing to hang my most used (and also very heavy) all-clad pan from the side of my fridge. The refrigerator had some sort of glossy powder coat and magnets hold well if you try to pull them straight off but end up sliding down anyway. This is where I came to appreciate the rubber shoe of this magnet. It protects my fridge but I think it plays an essential part in the grip strength of the magnet. I can not slide it around - I need to use two hands to pull it off then place it in another position.
To give a magnet strength example I had one magnetic hook on my desk as I was writing this review. My nearby desk-size scotch tape dispenser came sliding over and the magnet grabbed it. I was able to lift the tape dispense just by the magnet grabbing the little piece of metal serrated teeth that cut the tape. There is no other metal in my dispenser. That's it!
In summary, four stars because it REALLY grips and holds a lot of weight. Minus a star because I do think they are a little expensive, they required assembly without tools or instructions, and the hooks themselves are not adjustable. I have some similar magnetic hooks and you can move the two prongs apart or together depending on what your tool size is. These prongs are strong and firm and are not moving and this cuts down of the number of uses a little bit.
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Reviewed in the United States on March 12, 2026
★★★★★ 5
Develop some quality, worth-while hangups!
Style: Updated 3.46in Magnet L Red Hooks 2PCS, Style: Updated 3.46in Magnet L Red Hooks 2PCS
Organization is key for any shop space. This goes double for smaller shops. If you look around and DON'T see something that could benefit from getting off the floor and onto a peg or hook, I applaud you! For the rest of us, where can we get started? Here you go.
These hooks come two in a package. The quality of these are easy to see. The powder coating is very nice as powder coating is more durable than paint. The orange plastic coating on the hooks feels thick and durable. Assembly is easy. There's a threaded post sticking out of the black rubber coated magnet "puck" that you insert into a opening on the hook and secure with washers and a nut. I like that they included both a washer and locking washer. If it matters, I always stack the fastener in the following order: washer, locking washer, then nut. Using a 14mm socket, I was done in no time.
How's much will these hooks hold? The magnetic power on these hooks feels substantial. When I slowly approached the side of my metal toolbox, the magnet made an audible snap when it magnetized to the side. It felt strong! The bend at the base of the hook added stability pressing against the toolbox but didn't mark the surface since it was covered by plastic. It held my heavy 50a extension cable and the other hook held both my 25' and 50' pressure washer hoses. Neither moved at all and felt very secure.
Whether your shop is your garage or basement, making the most of your space makes a difference and these can help. These are very strong and hold items safely. I'm very happy having these around and will be getting more. If you have a metal surface that you can attach hooks, give these a try. It's a quality solution!
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Reviewed in the United States on March 21, 2026
★★★★★ 1
Not strong
Style: Updated 3.46in Magnet L Red Hooks 2PCS
The magnets are not strong enough to hold garden tools!
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Awesome product!!!
Style: Updated 3.46in Magnet L Red Hooks 2PCS, Style: Updated 3.46in Magnet L Red Hooks 2PCS
Wow when they describe these magnets as “super string” they are not joking! Magnets are so strong you must be careful when placing them on your metal cabinet, lest it pinch your finger when the magnet grabs the metal!
This is an awesome product. The hooks have a hole in their base that fits over a threaded post / bolt coming out of the center of the magnet and the kit comes with two washers, lock washers, and bolts to attach the hooks to the magnets. Takes about one minute per magnet to assemble. Once on the wall they feel like they will hold a lot of weight though I did not try to test the upper limit. No problem at all holding yard tools and the like. The hooks or arms have a nice rubberized coating and extend about 4.5” out from the wall. At $15 each these seemed a little pricy but wow what a great solution for hanging things on metal walls. They work so well I will be buying more of these! I actually have some other similar very strong round magnets made for a similar purpose … but they do not have the “built-in” hooks like these do and it makes a big difference for the usefulness! Considering all of that I think they are a great value. Item reviewed: MUTUACTOR Updated Super Strong Magnetic Hooks,40lbs, 2-pack
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Reviewed in the United States on March 28, 2026