SKU: 42518981885

Trypan Blue Staining Cell Viability Assay Kit

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Description

Trypan Blue Staining Cell Viability Assay KitProduct Specification Usage For reference only: 1. Collect cells: Take 100 L of resuspended cells into a plastic centrifuge tube, add 100 L of Trypan blue staining solution (2X), gently mix well, and stain for 3 minutes (staining for 3 minutes is sufficient, staining time can be longer, but should not exceed 10 minutes)3. Count: Take a small amount of stained cells and count them using a blood cell counting board. Usually, if precise quantification is

Product Specification

Usage

For reference only:
1. Collect cells:Take 100 μ L of resuspended cells into a plastic centrifuge tube, add 100 μ L of Trypan blue staining solution (2X), gently mix well, and stain for 3 minutes (staining for 3 minutes is sufficient, staining time can be longer, but should not exceed 10 minutes)3. Count:
Take a small amount of stained cells and count them using a blood cell counting board. Usually, if precise quantification is required, each cell sample should have up to a few 500 cells, counting the blue cells and the total number of cells. The formula for calculating cell survival rate is as follows:
Cell survival rate=(total number of cells - number of blue cells)/total number of cells× 100%

Description

  Trypan blue staining cell viability assay kit is developed by using normal healthy cells that can reject trypan blue, while cells that lose cell membrane integrity can be stained with trypan blue. Trypan blue staining detects the integrity of the cell membrane. It is generally considered that the cell has died if the cell membrane loses integrity
  After trypan blue staining, the cell survival rate can be accurately quantified by counting directly under the microscope or counting after taking pictures under the microscope. Please refer to figure 1 for HeLa cells after trypan blue staining
  Trypan blue staining can be completed in only 3-5 minutes, and the operation is very simple
  This kit is sufficient to detect 100 cell samples
product components:

Product number Name 100T 500T
A Trypan blue staining solution (2x) 10ml 50ml
B Cell resuspension 100ml 500ml
General Notes

1.   This product may appear a small amount of granular precipitation after long-term storage. It can be fully dissolved in water bath at 37 ℃ for about 10 minutes. After the precipitate is completely dissolved, it can be used normally
2.   Trypan blue is toxic to human body. Please be very careful during operation and pay attention to effective protection to avoid direct contact with human body or inhalation
3.   For your safety and health, please wear experimental clothing and disposable gloves and mask for operation

Storage Temp. 2-8 ℃, 1 year
Shipping Notes
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Exchange/Return Notes
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SKU: 42518981885

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4.3 ★★★★★
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regina
Battle Creek, US
★★★★★ 3
Not durable
Color: Black/White, Size: Large
If you have a dog who likes to pop things then this toy isn't it. Within 5 min she had this ball popped. It was made to sound like it was durable for this type of thing but it definitely is not so just be aware. Now if you have a dog who doesn't pop things easy then this would be a good toy
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 15, 2026
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Verified Purchase
Billie W
Belleville, US
★★★★★ 5
They last quite well, even out in weather.
Color: Black/White, Size: Medium
NY corgi loves these!
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Reviewed in the United States on May 31, 2026
J
Verified Purchase
Joyce S.
Port Orchard, US
★★★★★ 5
Dog can't pop it!
Color: Black/White, Size: Medium
Great toy would buy again!
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Reviewed in the United States on November 17, 2025
E
Verified Purchase
Elaine Laventure
Whiting, US
★★★★★ 4
WHAT I LEARNED
Color: U.S.A, Size: Medium
i AM NOT SURE THAT 4 STARS IS A FAIR SCORE, MY DOG IS AN AGGRESSIVE CHEWER, NEEDLESS TO SAY WITHIN 2 DAYS ALL THE TABS WERE OFF, BUT THERE WERE NUBS LEFT AND SHE CAN USE THAT. HERE IS A WHAT NOT TO DO, BRING IT TO THE DOG PARK, THEY ALL HAVE A BLAST BUT IF YOUR A BULLY/PIT MIX AND DECIDE TO SINK THEIR TEETH INTO IT, IT DOES DEFLAT. LESSONS LEARNED STILL A FUN TOY.
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Reviewed in the United States on May 13, 2026
J
Verified Purchase
Jedi and Waggy
Massapequa, US
★★★★★ 5
Sturdy ball with pump
Color: Black/White, Size: Medium
Thick material so it won't pop so easy. Comes with a hand pump and 2 ball needles. Tabs for the dog to carry in his mouth. Although my dog bites the whole thing to carry. Savage loves it!
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Reviewed in the United States on February 4, 2026

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