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Description
Mouse IL-33 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 400 pg/mL). Then dilute to the following concentrations: 400 pg/mL, 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 400pg/mL standard working solution into the first EP tube and mix thoroughly to make a 200pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Sensitivity | 1.84 pg/mL | |||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 33 (IL-33) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Interleukin 33 (IL-33) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 33 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-33 (IL-33), also known as NFHEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription. IL-33 is constitutively expressed in smooth muscle and airway epithelial cells. Following stimulation with IL1α or IL1β, it is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes. IL-33 shares structural and functional features with the IL-1 family of cytokines. It binds and signals through the IL-1RL1/ST2 receptor, activating NF-kappaB and MAP kinase. IL-33 induces the production of TH2 cell-associated cytokines, including IL-4, IL-5, and IL-13, and exerts a variety of inflammatory-related biological activities. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 6.25-400pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.3 ★★★★★
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Product Reviews
★★★★★ 5
Absolutely Fabulous for Any Age - Not To Be Missed
Whether you have a 15-step skincare regime or are extremely low maintenance in your skincare routine, this is a product that is an essential component to a healthy, radiant complexion. I’ll admit, my skincare game became fairly extensive as I approached 40. Prior to that, I had a solid day and night routine using 4-5 products.
After 40, my skin required more effort with a focus on hydration, prevention of damage from sun, stress, lack of sleep, etc., barrier building, and aging gracefully. At 55, people often comment on how nice my skin is and some people think I’m still in my 40s.
I owe that to a fantastic arsenal of skincare products rather than procedures (plus water, solid nutrition, and at least 8 hours of sleep per night). Nothing wrong with getting Botox or fillers, but I’m rather frugal and would rather focus on daily maintenance to ensure my skin looks as good as possible regardless of my age.
This milky toner is as good - and actually better - than brands at much higher price points. It’s thick (in a good way), provides instant hydration, and keeps my skin glowing for hours. I use it in the morning as part of my AM skincare routine, as part of my skin prep prior to doing my makeup, and every night in my PM routine.
Some people use milky toner as a setting spray (mix equal parts toner and distilled water into a spray bottle and lightly mist your face once your makeup is complete). I prefer a setting spray that provides staying power rather than additional hydration, but I have sprayed this mixture on my face if I feel dry and my skin looks parched. It feels really nice on a hot day, especially if you keep the mister cool in the fridge.
I have tried adding drops to my foundation, which is a “viral” trend, but I find this product to be a bit too thick. A thinner consistency like the Byoma Milky Toner works better for me if I want to create a more dewy foundation. I find that using this Thayers product as part of my skin prep prior to foundation along with using a dewy, hydrating foundation is better for me.
I love that this bottle is huge, affordable, easily obtained from Amazon, and incredibly effective. It’s earned its spot in my holy grail products for life.
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Reviewed in the United States on July 9, 2025
★★★★★ 4
Gentle Milky Cleanser That Mostly Delivers on Hydration
I picked up the Thayers Milky Hydrating Face Cleanser with Snow Mushroom while it was on sale because I was looking for a non-drying face wash.
Overall, I do like it. The texture is gentle and milky—it doesn’t foam up, feels non-irritating on my skin, and rinses off easily. I also paired it with the moisturizer from the same line (though I skipped the toner since I already use one my skin prefers).
It does a good job cleansing without stripping my skin too harshly, which I appreciate since I tend to lean dry. That said, I do notice a slight dryness after washing and drying my face, which surprised me a bit given the “hydrating” claim.
One other small downside is that the packaging was a bit difficult to open.
Still, overall it’s a gentle, easy-to-use cleanser that feels calming on the skin and doesn’t cause irritation.
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Leaves face feeling clean, soft, and smooth
Love this facial cleaner. I actually double cleanse my face. First I use an oil based makeup remover, then I cleanse with this face wash. It doesn’t strip my skin of moisture, and I’ve noticed less redness on my face after switching to this cleanser. The cleanser is light and leaves my face feeling clean, soft, and smooth. I would recommend to people who notice their face feeling “tight” or noticing redness after cleansing.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Great every day cleanser.
My go to facial cleanser. Mild and protects your skin barrier. Great for any skin type.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Sturdy & easy to move around
Its solid & sturdy. It does exactly what it’s supposed to. You have the option to fill it up with water or sand , I choose water. It holds the umbrella in place. The wheels and handle make it really easy to move around, which is a huge plus. It fits perfectly and feels very well made. Great addition for my pool area!
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Reviewed in the United States on April 18, 2026