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Description
PE-Cy7 Rat Anti-Human CD133 Antibody (S-R586)Product Specification Host Rat Antigen CD133 Synonyms Prominin 1; Antigen AC133; Prominin like protein 1; PROML1; PROM1 Location Cell membrane, Endoplasmic reticulum Accession O43490 Clone Number S R586 Antibody Type Rat mAb Isotype IgG1,k Application FCM Reactivity Hu Positive Sample human PBMC Purification Protein G Concentration 0. 2 mg ml Conjugation PE Cy7 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability &
Product Specification
| Host | Rat |
| Antigen | CD133 |
| Synonyms | Prominin-1; Antigen AC133; Prominin-like protein 1; PROML1; PROM1 |
| Location | Cell membrane, Endoplasmic reticulum |
| Accession | O43490 |
| Clone Number | S-R586 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD133, also known as prominin-1, is a pentaspan transmembrane glycoprotein encoded by the PROM1 gene. It is widely used as a biomarker for the isolation and identification of stem and progenitor cells in various normal tissues and cancer stem cells (CSCs) in different types of tumors. This 97-kDa protein has five transmembrane domains, with its N-terminus extending into the extracellular space and the C-terminus residing inside the cell. CD133 is expressed in neural progenitor cells, human hematopoietic cells, and various cancers. Its presence in cancer cells is often associated with poor prognosis, therapy resistance, and tumor metastasis.
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