SKU: 37795770240

Fixable Viability Dye 515

Sale price$94.50 Regular price$105.00
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Ships within 48 hours · Estimated delivery Jul 23 - Jul 28

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Description

Fixable Viability Dye 515Product Specification Stability & Storage 12 months from date of receipt, 20 C as supplied. Background Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC. Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this

Product Specification


Stability & Storage

12 months from date of receipt, -20 °C as supplied.

Background

Fixable Viability Dye 515 is excited by the Blue laser (with an excitation maximum of 488 nm) and has fluorescence emission maximum at 515 nm, Fixable Viability Dye 515 has similar emission to FITC.

Fixable Viability Dye 515 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.

Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

Protocol

1.Wash cells one time in PBS buffer.

2.Add 1 µl of Fixable Viability Dye 515 Stock Solution for each 1~2 ml of cell suspension (1:1000~1:2000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

3.Resuspend cells at 1*10^7 cells/ml in Fixable Viability Dye 515, Incubate the mixture for 10-15 minutes at room temperature protected from light.

4.Wash cells twice with PBS buffer or equivalent buffer containing serum or BSA.

5.Resuspend the cells in Stain Buffer.

Note: This protocol applicable to mouse splenocytes Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.

Shipping Notes
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Exchange/Return Notes
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SKU: 37795770240

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Verified Purchase
Dennis
Waukegan, US
★★★★★ 5
Good shoe inexpensive
Size: 11.5, Color: Black/White
Perfect. The fit and slip on ability. And it came in an actual shoe box.
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Reviewed in the United States on May 24, 2026
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Verified Purchase
Dawna Lee
Port Orchard, US
★★★★★ 5
Great shoes
Size: 13, Color: Black/White
Great shoes, bought for my grandson's birthday, he was very pleased with them. I'm very pleased that they came in many sizes.
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Reviewed in the United States on May 19, 2026
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Verified Purchase
David Zielinski
Omaha, US
★★★★★ 5
Great Value for the Money!
Size: 10.5, Color: Black/White
Pleasantly surprised at this price point! Comfy, sturdy and a way better sole the Sketchers! Amazon products in general have been really good quality at affordable prices!
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Reviewed in the United States on May 3, 2026
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Verified Purchase
Gaile Kausch
Lowell, US
★★★★★ 1
Size not as expected
Size: 11.5, Color: Grey
Ordered same size as I always do. Way too large. Size listed was off by a long shot
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Reviewed in the United States on May 22, 2026
P
Professor's opinion
Battle Creek, US
★★★★★ 4
Lightweight and True to Size — Best for Casual Use
Size: 9.5, Color: Navy, Size: 9.5, Color: Navy
It is nice to see the Amazon Essentials brand expand from household essentials to apparel and shoes. I ordered the Amazon Essentials Men's Lightweight Knit Running Sneakers. The product is made in Vietnam and comes in a shoe box, which is nice. I've noticed that quite often, inexpensive shoes are delivered just in a plastic bag. The sneakers are ready to use and have no odors from glue, organic solvents, etc., which is common nowadays. I wear a size 9W or 9.5. These sneakers are 9.5 and feel like a typical 9.5 size, without surprises. The product is advertised as lightweight, and it is true. Each shoe weighs below 0.6 lb (265 grams). Inside is soft memory foam; the shoes sit on the foot well, snug enough and comfortable, and are easy to put on and take off. They seem like a good fit for spring and summer as casual footwear or for light gym use. The sole is simple and not designed for performance or high-impact activities. Overall, a solid, reasonably priced, comfortable everyday option, though not intended for serious running or training.
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Reviewed in the United States on April 15, 2026

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